Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1030003..1030621 Replicon chromosome
Accession NZ_CP069480
Organism Escherichia coli strain FDAARGOS_1257

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J87_RS05080 Protein ID WP_001291435.1
Coordinates 1030403..1030621 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J87_RS05075 Protein ID WP_000344800.1
Coordinates 1030003..1030377 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J87_RS05065 1025093..1026286 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6J87_RS05070 1026309..1029458 + 3150 WP_001132481.1 multidrug efflux RND transporter permease subunit -
I6J87_RS05075 1030003..1030377 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J87_RS05080 1030403..1030621 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J87_RS05085 1030795..1031346 + 552 WP_000102539.1 maltose O-acetyltransferase -
I6J87_RS05090 1031462..1031932 + 471 WP_000136192.1 YlaC family protein -
I6J87_RS05095 1032096..1033646 + 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J87_RS05100 1033688..1034041 - 354 WP_000878138.1 DUF1428 family protein -
I6J87_RS05110 1034420..1034731 + 312 WP_000409908.1 MGMT family protein -
I6J87_RS05115 1034762..1035334 - 573 WP_000779830.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190583 WP_001291435.1 NZ_CP069480:1030403-1030621 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190583 NZ_CP091470:4588245-4588463 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCTGCGGCCGATCATCGTCTTGCAGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190583 WP_000344800.1 NZ_CP069480:1030003-1030377 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190583 NZ_CP091470:4587844-4588218 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGATCCAACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTTGCACTTAATTATAAAATTAAGTACGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References