Detailed information of TA system TA190570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 708936..709465
• Replicon: chromosome
• Accession: NZ_CP069470
• Organism: Staphylococcus aureus strain FDAARGOS_1246

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: I6J76_RS03540
• Protein ID: WP_000621175.1
• Coordinates: 709103..709465 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: I6J76_RS03535
• Protein ID: WP_000948331.1
• Coordinates: 708936..709106 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J76_RS03505	703973..704533	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
I6J76_RS03510	704741..705220	+	480	WP_001287088.1	hypothetical protein	-
I6J76_RS03515	705213..706796	+	1584	WP_001294626.1	PH domain-containing protein	-
I6J76_RS03520	706783..707274	+	492	WP_001205910.1	PH domain-containing protein	-
I6J76_RS03525	707278..707637	+	360	WP_000581200.1	holo-ACP synthase	-
I6J76_RS03530	707703..708851	+	1149	WP_001281145.1	alanine racemase	-
I6J76_RS03535	708936..709106	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
I6J76_RS03540	709103..709465	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6J76_RS03545	709814..710815	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
I6J76_RS03550	710934..711260	+	327	WP_001052491.1	anti-sigma factor antagonist	-
I6J76_RS03555	711262..711741	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
I6J76_RS03560	711716..712486	+	771	WP_001041103.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T190570 WP_000621175.1 NZ_CP069470:709103-709465 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T190570 NZ_CP091456:2202977-2203309 [Avibacterium sp. 20-132]
ATGAAAGCGGTTTTTATTGAATTGCCATTTTTTGAAAAATATAGAAAGGAATATCTCTCTGACGATGAGTATCTTATGCT
ACAAAATGAGTTACTTGTTGCACCAGAAAAAGGAGATTTAATACAAGGCACCGGTGGACTTAGGAAATTAAGAATTGCAA
ATTCTAAAAGAAATAGAGGTAAAAGGGGCGGAGCAAGAGTAATTTATTATTACTATGTGCGTAATGCCGCAATCTACTTT
TTAACCGCCTATGGTAAAGAGATGAAAGAAGATCTCACTGCTGATGAAAAAAGTATCTTGGCTAAAATAGTAGAAAATAT
AAAGGGGAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT190570 WP_000948331.1 NZ_CP069470:708936-709106 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT190570 NZ_CP091456:2203313-2203627 [Avibacterium sp. 20-132]
ATGGAAAGAAATTTATTTGAAGAATTGAAACAAGGATTGGAACAAATGCAAGTCCATTTGGAAGGTAAAACCACCCTCAA
AACCATTGAGCGTGAGCTTCCAGCAGAAATGGGTGAAATAAGCGCGAATGAAGTGAAGTCTATCCGTGAAAAACTTAATC
TATCGCAATCGGTTTTTGCTAAGAAATTGAGAATGAGCGTACGCACTTACCAAGGTTGGGAACAAGGCAAAACCACGCCA
AATAAACAAGCCACATTACTGTTGAAGATGATTGAGCGCTCGCCGCAAACCTTTGAAGATATTGCATCTCTCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1