Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 7608..8215 Replicon plasmid unnamed2
Accession NZ_CP069461
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name doc Uniprot ID B7LIH8
Locus tag I6J79_RS24630 Protein ID WP_000673985.1
Coordinates 7829..8215 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID B7LIH9
Locus tag I6J79_RS24625 Protein ID WP_000493532.1
Coordinates 7608..7832 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J79_RS24610 3778..4197 - 420 WP_000650705.1 polymer-forming cytoskeletal protein -
I6J79_RS24615 5354..6526 + 1173 Protein_7 IS21-like element IS21 family transposase -
I6J79_RS24620 6526..7323 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
I6J79_RS24625 7608..7832 + 225 WP_000493532.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6J79_RS24630 7829..8215 + 387 WP_000673985.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I6J79_RS24635 8993..10717 - 1725 WP_001554923.1 ABC transporter ATP-binding protein/permease -
I6J79_RS24640 10717..11400 - 684 WP_000200414.1 lasso peptide biosynthesis B2 protein -
I6J79_RS24645 11676..12788 - 1113 Protein_13 hypothetical protein -
I6J79_RS24650 12916..13104 - 189 WP_000744815.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - senB 1..114299 114299


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(11-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14370.62 Da        Isoelectric Point: 7.0732

>T190553 WP_000673985.1 NZ_CP069461:7829-8215 [Escherichia coli]
MKFVSSQEVIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYHYEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVA
QVFLKRNGVHIISSRISFDEMQIIALNAATGEYNWKMVSDHLKAIILN

Download         Length: 387 bp

>T190553 NZ_CP091428:c122165-121860 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8361.39 Da        Isoelectric Point: 5.6713

>AT190553 WP_000493532.1 NZ_CP069461:7608-7832 [Escherichia coli]
MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDAFERYRKAALDAEFAEIMQSHGTAYTELSKR

Download         Length: 225 bp

>AT190553 NZ_CP091428:c122385-122167 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086V9Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A086V9Y8

References