Detailed information of TA system TA190549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 10544..11069
• Replicon: plasmid unnamed1
• Accession: NZ_CP069460
• Organism: Escherichia coli strain FDAARGOS_1249

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: I6J79_RS24470
• Protein ID: WP_001159871.1
• Coordinates: 10764..11069 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H9TJP1
• Locus tag: I6J79_RS24465
• Protein ID: WP_000813630.1
• Coordinates: 10544..10762 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J79_RS24420	5629..5842	+	214	Protein_4	Tn3 family transposase	-
I6J79_RS24425	6242..6526	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	-
I6J79_RS24430	6526..6801	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6J79_RS24435	7137..7235	-	99	Protein_7	proQ/FINO family protein	-
I6J79_RS24440	7264..8232	+	969	Protein_8	IS5-like element IS903B family transposase	-
I6J79_RS24445	8274..8609	-	336	WP_089537698.1	colicin transporter	-
I6J79_RS24450	8738..9085	+	348	WP_000142455.1	hypothetical protein	-
I6J79_RS24455	9103..9693	-	591	WP_000194570.1	hypothetical protein	-
I6J79_RS24460	9693..9950	-	258	WP_000343085.1	hypothetical protein	-
I6J79_RS24465	10544..10762	+	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
I6J79_RS24470	10764..11069	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
I6J79_RS24475	11070..11876	+	807	WP_000016968.1	site-specific integrase	-
I6J79_RS24480	12556..13287	-	732	WP_000504263.1	replication initiation protein	-
I6J79_RS24485	13907..15112	+	1206	WP_001442122.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36471	36471
	-	flank	IS/Tn	-	-	7483..8232	749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T190549 WP_001159871.1 NZ_CP069460:10764-11069 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T190549 NZ_CP091427:c3953246-3952932 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8354.27 Da
• Isoelectric Point: 4.5203

>AT190549 WP_000813630.1 NZ_CP069460:10544-10762 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT190549 NZ_CP091427:c3952935-3952519 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A829CEF5