Detailed information of TA system TA190548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 6242..6801
• Replicon: plasmid unnamed1
• Accession: NZ_CP069460
• Organism: Escherichia coli strain FDAARGOS_1249

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: I6J79_RS24430
• Protein ID: WP_000421257.1
• Coordinates: 6526..6801 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: I6J79_RS24425
• Protein ID: WP_001178089.1
• Coordinates: 6242..6526 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J79_RS24405	1436..2839	+	1404	WP_016230922.1	FAD-dependent tricarballylate dehydrogenase TcuA	-
I6J79_RS24410	2826..3965	+	1140	WP_059328375.1	tricarballylate utilization 4Fe-4S protein TcuB	-
I6J79_RS24415	4020..5315	+	1296	WP_032190702.1	tricarballylate/proton symporter TcuC	-
I6J79_RS24420	5629..5842	+	214	Protein_4	Tn3 family transposase	-
I6J79_RS24425	6242..6526	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6J79_RS24430	6526..6801	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J79_RS24435	7137..7235	-	99	Protein_7	proQ/FINO family protein	-
I6J79_RS24440	7264..8232	+	969	Protein_8	IS5-like element IS903B family transposase	-
I6J79_RS24445	8274..8609	-	336	WP_089537698.1	colicin transporter	-
I6J79_RS24450	8738..9085	+	348	WP_000142455.1	hypothetical protein	-
I6J79_RS24455	9103..9693	-	591	WP_000194570.1	hypothetical protein	-
I6J79_RS24460	9693..9950	-	258	WP_000343085.1	hypothetical protein	-
I6J79_RS24465	10544..10762	+	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	-
I6J79_RS24470	10764..11069	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36471	36471
	-	flank	IS/Tn	-	-	7483..8232	749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T190548 WP_000421257.1 NZ_CP069460:6526-6801 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T190548 NZ_CP091427:c3888671-3888375 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT190548 WP_001178089.1 NZ_CP069460:6242-6526 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT190548 NZ_CP091427:c3888373-3887978 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A731IHA8