Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4433379..4434033 Replicon chromosome
Accession NZ_CP069459
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I6J79_RS21485 Protein ID WP_000244777.1
Coordinates 4433626..4434033 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6J79_RS21480 Protein ID WP_000354046.1
Coordinates 4433379..4433645 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J79_RS21455 4428548..4429291 + 744 WP_000951964.1 SDR family oxidoreductase -
I6J79_RS21460 4429348..4430781 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
I6J79_RS21465 4430826..4431137 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
I6J79_RS21470 4431301..4431960 + 660 WP_000250274.1 hemolysin III family protein -
I6J79_RS21475 4432156..4433136 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
I6J79_RS21480 4433379..4433645 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6J79_RS21485 4433626..4434033 + 408 WP_000244777.1 toxin CptA Toxin
I6J79_RS21490 4434073..4434594 - 522 WP_001055874.1 flavodoxin FldB -
I6J79_RS21495 4434706..4435602 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6J79_RS21500 4435627..4436337 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6J79_RS21505 4436343..4438076 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T190546 WP_000244777.1 NZ_CP069459:4433626-4434033 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T190546 NZ_CP091427:c3681564-3681157 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT190546 WP_000354046.1 NZ_CP069459:4433379-4433645 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT190546 NZ_CP091427:c3681811-3681545 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References