Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2170165..2170783 Replicon chromosome
Accession NZ_CP069459
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J79_RS10760 Protein ID WP_001291435.1
Coordinates 2170565..2170783 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J79_RS10755 Protein ID WP_000344800.1
Coordinates 2170165..2170539 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J79_RS10745 2165253..2166446 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6J79_RS10750 2166469..2169618 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6J79_RS10755 2170165..2170539 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J79_RS10760 2170565..2170783 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J79_RS10765 2170955..2171506 + 552 WP_000102564.1 maltose O-acetyltransferase -
I6J79_RS10770 2171622..2172092 + 471 WP_000136192.1 YlaC family protein -
I6J79_RS10775 2172256..2173806 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J79_RS10780 2173848..2174201 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6J79_RS10790 2174580..2174891 + 312 WP_000409911.1 MGMT family protein -
I6J79_RS10795 2174922..2175494 - 573 WP_193013556.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190539 WP_001291435.1 NZ_CP069459:2170565-2170783 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190539 NZ_CP091427:c1923051-1922944 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190539 WP_000344800.1 NZ_CP069459:2170165-2170539 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190539 NZ_CP091427:1923098-1923164 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References