Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 928762..929288 Replicon chromosome
Accession NZ_CP069459
Organism Escherichia coli strain FDAARGOS_1249

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag I6J79_RS04410 Protein ID WP_000323025.1
Coordinates 928762..929049 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag I6J79_RS04415 Protein ID WP_000534858.1
Coordinates 929049..929288 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J79_RS04370 923958..924170 + 213 WP_000087756.1 cold shock-like protein CspF -
I6J79_RS04375 924225..924314 + 90 WP_120795389.1 hypothetical protein -
I6J79_RS04380 924592..925344 - 753 WP_001047135.1 antitermination protein -
I6J79_RS04385 925358..926407 - 1050 WP_001265199.1 DUF968 domain-containing protein -
I6J79_RS04390 926409..926687 - 279 WP_032351280.1 hypothetical protein -
I6J79_RS04395 926754..927005 - 252 WP_000980994.1 hypothetical protein -
I6J79_RS04400 927222..927359 - 138 WP_044703989.1 type I toxin-antitoxin system Hok family toxin -
I6J79_RS04405 927426..928639 + 1214 WP_204147805.1 IS3 family transposase -
I6J79_RS04410 928762..929049 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
I6J79_RS04415 929049..929288 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
I6J79_RS04420 929313..929618 + 306 WP_001326990.1 hypothetical protein -
I6J79_RS04425 929821..930153 + 333 WP_001301033.1 protein FlxA -
I6J79_RS04430 930590..931903 - 1314 Protein_876 ISNCY family transposase -
I6J79_RS04435 932672..932959 - 288 Protein_877 hypothetical protein -
I6J79_RS04440 933570..933926 - 357 WP_001310834.1 class I SAM-dependent methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 890903..947777 56874


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T190538 WP_000323025.1 NZ_CP069459:c929049-928762 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T190538 NZ_CP091427:c1922516-1922409 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT190538 WP_000534858.1 NZ_CP069459:c929288-929049 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT190538 NZ_CP091427:1922563-1922629 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References