Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 93004..93529 Replicon plasmid unnamed1
Accession NZ_CP069454
Organism Escherichia coli strain FDAARGOS_1254

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I6J84_RS22840 Protein ID WP_001159871.1
Coordinates 93224..93529 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6J84_RS22835 Protein ID WP_000813634.1
Coordinates 93004..93222 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J84_RS22830 90041..90682 - 642 WP_000990667.1 hypothetical protein -
I6J84_RS22835 93004..93222 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6J84_RS22840 93224..93529 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I6J84_RS22845 93530..94339 + 810 WP_000016979.1 site-specific integrase -
I6J84_RS22850 94477..94752 - 276 WP_000239529.1 hypothetical protein -
I6J84_RS22855 94746..95390 - 645 WP_000633911.1 AAA family ATPase -
I6J84_RS22860 95619..96590 + 972 WP_001103694.1 hypothetical protein -
I6J84_RS22865 96595..96987 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
I6J84_RS22870 96992..98262 - 1271 Protein_109 translesion error-prone DNA polymerase V subunit UmuC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114230 114230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T190523 WP_001159871.1 NZ_CP069454:93224-93529 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T190523 NZ_CP091423:c1975469-1975212 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCCAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT190523 WP_000813634.1 NZ_CP069454:93004-93222 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT190523 NZ_CP091423:c1975731-1975471 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References