Detailed information of TA system TA190521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4392993..4393583
• Replicon: chromosome
• Accession: NZ_CP069453
• Organism: Escherichia coli strain FDAARGOS_1254

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V0VE57
• Locus tag: I6J84_RS21080
• Protein ID: WP_000373724.1
• Coordinates: 4392993..4393325 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: I6J84_RS21085
• Protein ID: WP_000288812.1
• Coordinates: 4393326..4393583 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J84_RS21055	4388456..4389718	+	1263	WP_000055690.1	integrase arm-type DNA-binding domain-containing protein	-
I6J84_RS21060	4389857..4390315	-	459	WP_000526101.1	IS200/IS605 family transposase	-
I6J84_RS21065	4391267..4392145	-	879	WP_000511773.1	integrase domain-containing protein	-
I6J84_RS21070	4392283..4392609	+	327	Protein_4100	hypothetical protein	-
I6J84_RS21075	4392590..4392946	+	357	WP_001147230.1	hypothetical protein	-
I6J84_RS21080	4392993..4393325	-	333	WP_000373724.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6J84_RS21085	4393326..4393583	-	258	WP_000288812.1	antitoxin	Antitoxin
I6J84_RS21090	4393681..4393872	+	192	WP_077561361.1	hypothetical protein	-
I6J84_RS21095	4393931..4394137	-	207	WP_000983730.1	helix-turn-helix domain-containing protein	-
I6J84_RS21100	4394134..4394601	-	468	WP_000201267.1	hypothetical protein	-
I6J84_RS21105	4394834..4395970	-	1137	WP_000420971.1	ISAs1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4378243..4423514	45271
	-	inside	IScluster/Tn	-	-	4389857..4395970	6113

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11724.57 Da
• Isoelectric Point: 10.5834

>T190521 WP_000373724.1 NZ_CP069453:c4393325-4392993 [Escherichia coli]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T190521 NZ_CP091423:c1348520-1348251 [Streptococcus suis]
ATGGCTTATAAGCTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAATTTGGGAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTAATTTGTGATATTATTGATAACAAAATGGTCATCCTGGCCCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT190521 WP_000288812.1 NZ_CP069453:c4393583-4393326 [Escherichia coli]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT190521 NZ_CP091423:c1348737-1348510 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGATTAAACGATGCAGAAGAAGAAATTTTCAGAAGCTATGCTATTCATACAGGAAAAAGCCT
GTCTGAGCTATTCAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Structures

Toxin

• AlphaFold DB: V0VE57

Antitoxin

• AlphaFold DB: A0A3S7DDD0