Detailed information of TA system TA190512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3920329..3921917
• Replicon: chromosome
• Accession: NZ_CP069453
• Organism: Escherichia coli strain FDAARGOS_1254

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I6J84_RS18690
• Protein ID: WP_001125428.1
• Coordinates: 3920329..3921651 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: I6J84_RS18695
• Protein ID: WP_001304368.1
• Coordinates: 3921651..3921917 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J84_RS18660	3915448..3916512	-	1065	WP_000859696.1	alpha/beta fold hydrolase	-
I6J84_RS18665	3916670..3917431	-	762	WP_001066620.1	carboxymuconolactone decarboxylase family protein	-
I6J84_RS18670	3917409..3917887	-	479	Protein_3631	cupin domain-containing protein	-
I6J84_RS18675	3918276..3918623	-	348	WP_001337832.1	hypothetical protein	-
I6J84_RS18680	3918649..3919119	-	471	Protein_3633	AEC family transporter	-
I6J84_RS18685	3919662..3920075	+	414	WP_042115247.1	acetylxylan esterase	-
I6J84_RS18690	3920329..3921651	-	1323	WP_001125428.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6J84_RS18695	3921651..3921917	-	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6J84_RS18700	3922225..3922983	+	759	WP_001286543.1	trans-aconitate 2-methyltransferase	-
I6J84_RS18705	3922987..3923901	-	915	WP_001313828.1	bestrophin family inner membrane protein	-
I6J84_RS18710	3924098..3925549	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	3900464..3921935	21471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49331.98 Da
• Isoelectric Point: 8.1297

>T190512 WP_001125428.1 NZ_CP069453:c3921651-3920329 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
FRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T190512 NZ_CP091421:c1211174-1210428 [Bacillus sp. R45]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
AGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGGACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT190512 WP_001304368.1 NZ_CP069453:c3921917-3921651 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT190512 NZ_CP091421:c1210427-1210257 [Bacillus sp. R45]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: V0VAH2