Detailed information of TA system TA190491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 57235..57794
• Replicon: plasmid unnamed
• Accession: NZ_CP069452
• Organism: Escherichia coli strain FDAARGOS_1256

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: I6J86_RS24430
• Protein ID: WP_000421257.1
• Coordinates: 57519..57794 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: I6J86_RS24425
• Protein ID: WP_001178087.1
• Coordinates: 57235..57519 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J86_RS24405	52269..52889	-	621	WP_000176497.1	DUF1449 family protein	-
I6J86_RS24410	53156..53818	-	663	WP_000154142.1	hypothetical protein	-
I6J86_RS24415	53959..54600	-	642	WP_000335849.1	transcription termination factor NusG	-
I6J86_RS24420	55301..56332	+	1032	WP_000907881.1	replication initiation protein	-
I6J86_RS24425	57235..57519	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
I6J86_RS24430	57519..57794	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J86_RS24435	57904..58185	+	282	WP_087880289.1	hypothetical protein	-
I6J86_RS24440	58376..58642	-	267	WP_000627831.1	hypothetical protein	-
I6J86_RS24445	58693..60264	-	1572	WP_042096620.1	IS66 family transposase	-
I6J86_RS24450	60284..60631	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
I6J86_RS24455	60631..61281	-	651	WP_021565159.1	IS66 family insertion sequence hypothetical protein	-
I6J86_RS24460	61492..62094	-	603	WP_000517694.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / dfrA1 / ant(3'')-Ia / tet(B)	-	1..109644	109644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T190491 WP_000421257.1 NZ_CP069452:57519-57794 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T190491 NZ_CP091371:c2665367-2665185 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT190491 WP_001178087.1 NZ_CP069452:57235-57519 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT190491 NZ_CP091371:c2665120-2664716 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625