Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4168426..4169080 Replicon chromosome
Accession NZ_CP069451
Organism Escherichia coli strain FDAARGOS_1256

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag I6J86_RS19715 Protein ID WP_000244765.1
Coordinates 4168426..4168833 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6J86_RS19720 Protein ID WP_000354046.1
Coordinates 4168814..4169080 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J86_RS19695 4164383..4166116 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
I6J86_RS19700 4166122..4166832 - 711 WP_000715229.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6J86_RS19705 4166857..4167753 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6J86_RS19710 4167865..4168386 + 522 WP_001055874.1 flavodoxin FldB -
I6J86_RS19715 4168426..4168833 - 408 WP_000244765.1 protein YgfX Toxin
I6J86_RS19720 4168814..4169080 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6J86_RS19725 4169323..4170303 + 981 WP_000886080.1 tRNA-modifying protein YgfZ -
I6J86_RS19730 4170380..4171039 - 660 WP_000250274.1 hemolysin III family protein -
I6J86_RS19735 4171203..4171514 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
I6J86_RS19740 4171559..4172992 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T190486 WP_000244765.1 NZ_CP069451:c4168833-4168426 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T190486 NZ_CP091368:c1115487-1115098 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT190486 WP_000354046.1 NZ_CP069451:c4169080-4168814 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT190486 NZ_CP091368:c1115751-1115494 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References