Detailed information of TA system TA190483

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3184600..3185431
• Replicon: chromosome
• Accession: NZ_CP069451
• Organism: Escherichia coli strain FDAARGOS_1256

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: I6J86_RS15185
• Protein ID: WP_000854814.1
• Coordinates: 3185057..3185431 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6J86_RS15180
• Protein ID: WP_024174331.1
• Coordinates: 3184600..3184968 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J86_RS15145	3179657..3180727	+	1071	WP_000102631.1	patatin-like phospholipase family protein	-
I6J86_RS15150	3180863..3181540	+	678	WP_001362823.1	hypothetical protein	-
I6J86_RS15155	3181556..3181966	+	411	WP_000846704.1	hypothetical protein	-
I6J86_RS15160	3182187..3183008	+	822	WP_001234571.1	DUF945 domain-containing protein	-
I6J86_RS15165	3183271..3183744	+	474	WP_000855074.1	antirestriction protein	-
I6J86_RS15170	3183760..3184236	+	477	WP_001541535.1	RadC family protein	-
I6J86_RS15175	3184305..3184526	+	222	WP_000692346.1	DUF987 domain-containing protein	-
I6J86_RS15180	3184600..3184968	+	369	WP_024174331.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J86_RS15185	3185057..3185431	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
I6J86_RS15190	3185428..3185622	+	195	WP_000988601.1	hypothetical protein	-
I6J86_RS15195	3185635..3185748	+	114	WP_001161660.1	DUF957 domain-containing protein	-
I6J86_RS15200	3186037..3186210	-	174	WP_032082634.1	transposase domain-containing protein	-
I6J86_RS15205	3186237..3186419	+	183	WP_001016348.1	hypothetical protein	-
I6J86_RS15210	3186520..3186849	-	330	WP_000450409.1	DUF496 family protein	-
I6J86_RS15215	3187021..3188079	-	1059	WP_001200890.1	FUSC family protein	-
I6J86_RS15220	3188277..3188750	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
I6J86_RS15225	3188869..3190035	-	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T190483 WP_000854814.1 NZ_CP069451:3185057-3185431 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T190483 NZ_CP091366:c2721409-2721227 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13603.42 Da
• Isoelectric Point: 6.7386

>AT190483 WP_024174331.1 NZ_CP069451:3184600-3184968 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Nucleotide

• Length: 369 bp

>AT190483 NZ_CP091366:c2721162-2720758 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Toxin

• AlphaFold DB: A0A829FY50