Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 107225..107750 Replicon plasmid unnamed1
Accession NZ_CP069448
Organism Escherichia coli strain FDAARGOS_1255

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag I6J85_RS24155 Protein ID WP_001159871.1
Coordinates 107225..107530 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag I6J85_RS24160 Protein ID WP_000813634.1
Coordinates 107532..107750 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J85_RS24125 102492..103762 + 1271 Protein_121 translesion error-prone DNA polymerase V subunit UmuC -
I6J85_RS24130 103767..104159 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
I6J85_RS24135 104164..105135 - 972 WP_001103694.1 hypothetical protein -
I6J85_RS24140 105364..106008 + 645 WP_000633911.1 AAA family ATPase -
I6J85_RS24145 106002..106277 + 276 WP_000239529.1 hypothetical protein -
I6J85_RS24150 106415..107224 - 810 WP_042091752.1 site-specific integrase -
I6J85_RS24155 107225..107530 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
I6J85_RS24160 107532..107750 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6J85_RS24165 110072..110713 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..114237 114237


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T190458 WP_001159871.1 NZ_CP069448:c107530-107225 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T190458 NZ_CP091356:c3014301-3014020 [Acinetobacter baumannii]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT190458 WP_000813634.1 NZ_CP069448:c107750-107532 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT190458 NZ_CP091356:c3014604-3014302 [Acinetobacter baumannii]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References