Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3887928..3888546 Replicon chromosome
Accession NZ_CP069447
Organism Escherichia coli strain FDAARGOS_1255

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J85_RS18535 Protein ID WP_001291435.1
Coordinates 3888328..3888546 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J85_RS18530 Protein ID WP_000344800.1
Coordinates 3887928..3888302 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J85_RS18520 3883018..3884211 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6J85_RS18525 3884234..3887383 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
I6J85_RS18530 3887928..3888302 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J85_RS18535 3888328..3888546 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J85_RS18540 3888719..3889270 + 552 WP_000102539.1 maltose O-acetyltransferase -
I6J85_RS18545 3889386..3889856 + 471 WP_001304825.1 YlaC family protein -
I6J85_RS18550 3890020..3891570 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J85_RS18555 3891612..3891965 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6J85_RS18565 3892344..3892655 + 312 WP_000409908.1 MGMT family protein -
I6J85_RS18570 3892686..3893258 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190449 WP_001291435.1 NZ_CP069447:3888328-3888546 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190449 NZ_CP091351:3810213-3810395 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190449 WP_000344800.1 NZ_CP069447:3887928-3888302 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190449 NZ_CP091351:3810488-3810892 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References