Detailed information of TA system TA190432

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 411875..412666
• Replicon: chromosome
• Accession: NZ_CP069447
• Organism: Escherichia coli strain FDAARGOS_1255

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: I6J85_RS01950
• Protein ID: WP_000691791.1
• Coordinates: 411875..412300 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L1KVZ6
• Locus tag: I6J85_RS01955
• Protein ID: WP_000939437.1
• Coordinates: 412331..412666 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J85_RS01925	407541..408038	-	498	WP_000509808.1	hypothetical protein	-
I6J85_RS01930	408720..409106	-	387	WP_001270938.1	DUF2441 domain-containing protein	-
I6J85_RS01935	409729..410460	-	732	WP_001216639.1	TIGR02646 family protein	-
I6J85_RS01940	410457..410678	-	222	WP_125112077.1	hypothetical protein	-
I6J85_RS01945	410663..411854	-	1192	Protein_365	AAA family ATPase	-
I6J85_RS01950	411875..412300	-	426	WP_000691791.1	TA system toxin CbtA family protein	Toxin
I6J85_RS01955	412331..412666	-	336	WP_000939437.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J85_RS01960	412666..413139	-	474	WP_001292742.1	DNA repair protein RadC	-
I6J85_RS01965	413169..413983	-	815	Protein_369	DUF945 domain-containing protein	-
I6J85_RS01970	414219..415172	-	954	WP_000290406.1	hypothetical protein	-
I6J85_RS01975	415765..416379	+	615	WP_000772910.1	inovirus Gp2 family protein	-
I6J85_RS01980	416497..416715	+	219	WP_000070772.1	AlpA family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	409729..432831	23102

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 16490.11 Da
• Isoelectric Point: 10.2376

>T190432 WP_000691791.1 NZ_CP069447:c412300-411875 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVNAVNFLVEKYELVRIDRKGF
SWQEQTPYLRAVDILRARQATGLRIQCKNTNIISRMADSRHDKRLYQRTFKACKRCYYTKT

Nucleotide

• Length: 426 bp

>T190432 NZ_CP091342:c399986-399804 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12556.56 Da
• Isoelectric Point: 7.6977

>AT190432 WP_000939437.1 NZ_CP069447:c412666-412331 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT190432 NZ_CP091342:c399711-399307 [Acinetobacter baumannii]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Antitoxin

• AlphaFold DB: A0A3L1KVZ6