Detailed information of TA system TA190430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 19735..20257
• Replicon: plasmid unnamed
• Accession: NZ_CP069446
• Organism: Escherichia coli strain FDAARGOS_1251

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: I6J81_RS24935
• Protein ID: WP_000220560.1
• Coordinates: 19735..20016 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: I6J81_RS24940
• Protein ID: WP_000121743.1
• Coordinates: 20006..20257 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J81_RS24885	15584..16750	-	1167	WP_000539529.1	relaxase/mobilization nuclease domain-containing protein	-
I6J81_RS24890	16753..17298	-	546	WP_000757693.1	hypothetical protein	-
I6J81_RS24895	17293..17367	-	75	Protein_19	DNA distortion polypeptide 3	-
I6J81_RS24900	17623..17895	-	273	WP_000160399.1	hypothetical protein	-
I6J81_RS24905	17908..18057	-	150	WP_000003880.1	hypothetical protein	-
I6J81_RS24910	18127..18306	-	180	WP_001550458.1	hypothetical protein	-
I6J81_RS24915	18349..18678	-	330	WP_000866648.1	hypothetical protein	-
I6J81_RS24920	18771..18986	-	216	WP_001180116.1	hypothetical protein	-
I6J81_RS24925	18976..19221	-	246	WP_001550454.1	hypothetical protein	-
I6J81_RS24930	19266..19589	-	324	WP_001181903.1	hypothetical protein	-
I6J81_RS24935	19735..20016	-	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6J81_RS24940	20006..20257	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
I6J81_RS24945	21319..22173	+	855	WP_001239324.1	replication initiation protein	-
I6J81_RS24950	22198..22644	+	447	WP_001074386.1	hypothetical protein	-
I6J81_RS24955	22712..23137	+	426	WP_001423344.1	hypothetical protein	-
I6J81_RS24960	23189..23422	-	234	WP_024252801.1	YcxB family protein	-
I6J81_RS24965	23600..23785	-	186	Protein_33	YcxB family protein	-
I6J81_RS24970	24220..24504	-	285	WP_001185480.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36124	36124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T190430 WP_000220560.1 NZ_CP069446:c20016-19735 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T190430 NZ_CP091340:432460-432849 [Acinetobacter baumannii]
TTGCTCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATAAGCTTTATTTTCTTTT
TAAAGCGTCCGCAACTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT190430 WP_000121743.1 NZ_CP069446:c20257-20006 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT190430 NZ_CP091340:432196-432453 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301