Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3936155..3936652 Replicon chromosome
Accession NZ_CP069445
Organism Escherichia coli strain FDAARGOS_1251

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6J81_RS18665 Protein ID WP_001173343.1
Coordinates 3936479..3936652 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6J81_RS18660 Protein ID WP_001312331.1
Coordinates 3936155..3936451 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J81_RS18640 3932883..3933602 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6J81_RS18645 3933599..3935230 - 1632 WP_001216466.1 sensor histidine kinase -
I6J81_RS18650 3935411..3935641 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
I6J81_RS18655 3935653..3935925 + 273 WP_000405647.1 N-acetyltransferase -
- 3936155..3936451 + 297 - - Antitoxin
I6J81_RS18660 3936155..3936451 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6J81_RS18665 3936479..3936652 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6J81_RS18670 3936771..3938288 - 1518 WP_000003805.1 lysine--tRNA ligase -
I6J81_RS18675 3938525..3939982 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T190422 WP_001173343.1 NZ_CP069445:3936479-3936652 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T190422 NZ_CP091333:1412-1801 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTCCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGTGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAAAACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT190422 WP_001312331.1 NZ_CP069445:3936155-3936451 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT190422 NZ_CP091333:1148-1405 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References