Detailed information of TA system TA190420

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3378525..3379323
• Replicon: chromosome
• Accession: NZ_CP069445
• Organism: Escherichia coli strain FDAARGOS_1251

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: I6J81_RS16090
• Protein ID: WP_001609485.1
• Coordinates: 3378946..3379323 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: I6J81_RS16085
• Protein ID: WP_001609483.1
• Coordinates: 3378525..3378899 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J81_RS16045	3373683..3374363	+	681	WP_001608660.1	WYL domain-containing protein	-
I6J81_RS16050	3374624..3374902	+	279	WP_001545361.1	hypothetical protein	-
I6J81_RS16055	3375241..3375552	+	312	WP_001608658.1	hypothetical protein	-
I6J81_RS16060	3375651..3376469	+	819	WP_001609564.1	DUF945 domain-containing protein	-
I6J81_RS16065	3376551..3377030	+	480	WP_001609479.1	antirestriction protein	-
I6J81_RS16070	3377046..3377522	+	477	WP_001186188.1	RadC family protein	-
I6J81_RS16075	3377591..3377812	+	222	WP_001609480.1	DUF987 domain-containing protein	-
I6J81_RS16080	3377831..3378475	+	645	WP_001609482.1	hypothetical protein	-
I6J81_RS16085	3378525..3378899	+	375	WP_001609483.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J81_RS16090	3378946..3379323	+	378	WP_001609485.1	TA system toxin CbtA family protein	Toxin
I6J81_RS16095	3379320..3379808	+	489	WP_001609486.1	hypothetical protein	-
I6J81_RS16100	3379820..3380017	+	198	WP_001609487.1	DUF957 domain-containing protein	-
I6J81_RS16105	3380113..3380957	+	845	Protein_3165	DUF4942 domain-containing protein	-
I6J81_RS16110	3381550..3382047	+	498	WP_000509811.1	hypothetical protein	-
I6J81_RS16115	3382214..3383137	+	924	WP_000535950.1	carboxylate/amino acid/amine transporter	-
I6J81_RS16120	3383141..3383959	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3367003..3382047	15044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.23 Da
• Isoelectric Point: 7.2141

>T190420 WP_001609485.1 NZ_CP069445:3378946-3379323 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T190420 NZ_CP091332:c56834-56529 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13675.54 Da
• Isoelectric Point: 6.4603

>AT190420 WP_001609483.1 NZ_CP069445:3378525-3378899 [Escherichia coli]
VSDTLPGTTLPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLKLMLTSG
ELNPCHQHTVTLYAKGLTCEADTFGSCGYVYLAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT190420 NZ_CP091332:c57054-56836 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA