Detailed information of TA system TA190418

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2559216..2560048
• Replicon: chromosome
• Accession: NZ_CP069445
• Organism: Escherichia coli strain FDAARGOS_1251

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: I6J81_RS12135
• Protein ID: WP_000854753.1
• Coordinates: 2559674..2560048 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: I6J81_RS12130
• Protein ID: WP_001278232.1
• Coordinates: 2559216..2559584 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J81_RS12100	2556052..2556507	+	456	WP_000581504.1	hypothetical protein	-
I6J81_RS12105	2556586..2556819	+	234	WP_001119729.1	DUF905 family protein	-
I6J81_RS12110	2556919..2557737	+	819	WP_001520163.1	DUF945 domain-containing protein	-
I6J81_RS12115	2557792..2558277	+	486	WP_001520165.1	antirestriction protein	-
I6J81_RS12120	2558293..2558769	+	477	WP_001186726.1	RadC family protein	-
I6J81_RS12125	2558832..2559053	+	222	WP_000692316.1	DUF987 domain-containing protein	-
I6J81_RS12130	2559216..2559584	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6J81_RS12135	2559674..2560048	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
I6J81_RS12140	2560045..2560533	+	489	WP_000777541.1	hypothetical protein	-
I6J81_RS12145	2560545..2560742	+	198	WP_000839281.1	DUF957 domain-containing protein	-
I6J81_RS12150	2560839..2561408	+	570	WP_001290241.1	DUF4942 domain-containing protein	-
I6J81_RS12155	2561750..2561920	+	171	Protein_2388	IS110 family transposase	-
I6J81_RS12160	2562731..2563714	+	984	WP_001616958.1	KpsF/GutQ family sugar-phosphate isomerase	-
I6J81_RS12165	2563786..2564934	+	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T190418 WP_000854753.1 NZ_CP069445:2559674-2560048 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T190418 NZ_CP091331:39566-39982 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT190418 WP_001278232.1 NZ_CP069445:2559216-2559584 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT190418 NZ_CP091331:39339-39569 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: E3PJ72