Detailed information of TA system TA190402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 14033..15647
• Replicon: plasmid unnamed
• Accession: NZ_CP069443
• Organism: Chromobacterium violaceum strain FDAARGOS_1274

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I6K04_RS22640
• Protein ID: WP_204086889.1
• Coordinates: 14298..15647 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6K04_RS22635
• Protein ID: WP_048404587.1
• Coordinates: 14033..14296 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6K04_RS22620	9925..10833	-	909	WP_204086886.1	TniB family NTP-binding protein	-
I6K04_RS22625	10836..12515	-	1680	WP_204086887.1	DDE-type integrase/transposase/recombinase	-
I6K04_RS22630	12707..13330	+	624	WP_204086888.1	recombinase family protein	-
I6K04_RS22635	14033..14296	+	264	WP_048404587.1	helix-turn-helix domain-containing protein	Antitoxin
I6K04_RS22640	14298..15647	+	1350	WP_204086889.1	type II toxin-antitoxin system HipA family toxin	Toxin
I6K04_RS22645	15956..17137	-	1182	WP_204086890.1	replication initiator protein A	-
I6K04_RS22650	17503..18330	+	828	WP_043616644.1	AAA family ATPase	-
I6K04_RS22655	18314..19219	+	906	WP_043616641.1	ParB/RepB/Spo0J family partition protein	-
I6K04_RS22660	19243..20193	+	951	WP_204086891.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49107	49107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (157-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-65)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 49917.03 Da
• Isoelectric Point: 8.1879

>T190402 WP_204086889.1 NZ_CP069443:14298-15647 [Chromobacterium violaceum]
MGRRKQQQKLHVWMNGLPVGSWETGRAGDRLTYFDDWVAHPQGRPLSLSLPFLPGGMPHQGAAVAAFFDNLLPDSEAIRR
RLAQRHRLAETSPFQLLSALGRDCVGAIQLLPEDETPRDLRTIRGEALDEASIARLLRQAVAAEPLGLREDEADLRLSIA
GAQEKTALLRHAGRWLLPRDSTPTTHIFKLPLGLVGGMQADMRSSVENEWLCSRLVAGFGLPVAACQIARFEEQKVLVVE
RFDRRLSADGSWILRQPQEDMCQATATPPHLKYQADGGPGIARILDILDGSQARERDRMDFFRAQLVFWLLAATDGHAKN
FSLFHLPGGQYQATPLYDILSTHPIIGNGSGRIAPQKVKLAMAVRGRNNHYLLERIRYSHWLAQARQVGLAPGQTARWID
ELVEQTDAVVQTVAKELPADFPQDVADAIFQGMRRQRDKLAAGKLAGDE

Nucleotide

• Length: 1350 bp

>T190402 NZ_CP091328:451932-452321 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9334.63 Da
• Isoelectric Point: 8.5556

>AT190402 WP_048404587.1 NZ_CP069443:14033-14296 [Chromobacterium violaceum]
MQYPVRTLEQLRPLLLGLRKAAGLTQTQLAARLGVSQQSYARLEANPAAASVERLYRALRALDAELALSSPGSPPPPSAA
DAHGEEW

Nucleotide

• Length: 264 bp

>AT190402 NZ_CP091328:451668-451925 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA