Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4039742..4040360 Replicon chromosome
Accession NZ_CP069441
Organism Escherichia coli strain FDAARGOS_1261

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6J91_RS18995 Protein ID WP_001291435.1
Coordinates 4039742..4039960 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6J91_RS19000 Protein ID WP_000344800.1
Coordinates 4039986..4040360 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J91_RS18960 4035030..4035602 + 573 WP_000779844.1 YbaY family lipoprotein -
I6J91_RS18965 4035633..4035944 - 312 WP_000409908.1 MGMT family protein -
I6J91_RS18975 4036323..4036676 + 354 WP_000878142.1 DUF1428 family protein -
I6J91_RS18980 4036718..4038268 - 1551 WP_001468081.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6J91_RS18985 4038432..4038902 - 471 WP_000136192.1 YlaC family protein -
I6J91_RS18990 4039018..4039569 - 552 WP_000102539.1 maltose O-acetyltransferase -
I6J91_RS18995 4039742..4039960 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6J91_RS19000 4039986..4040360 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6J91_RS19005 4040905..4044054 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6J91_RS19010 4044077..4045270 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190400 WP_001291435.1 NZ_CP069441:c4039960-4039742 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190400 NZ_CP091327:c86353-85910 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190400 WP_000344800.1 NZ_CP069441:c4040360-4039986 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190400 NZ_CP091327:c86580-86350 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References