Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3604017..3604567 Replicon chromosome
Accession NZ_CP069441
Organism Escherichia coli strain FDAARGOS_1261

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag I6J91_RS16980 Protein ID WP_001160966.1
Coordinates 3604253..3604567 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag I6J91_RS16975 Protein ID WP_000125566.1
Coordinates 3604017..3604250 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J91_RS16950 3599090..3599377 + 288 WP_000203741.1 ferredoxin-like protein FixX -
I6J91_RS16955 3599436..3600767 + 1332 WP_001183207.1 MFS transporter -
I6J91_RS16960 3600875..3601405 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
I6J91_RS16965 3601398..3603260 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
I6J91_RS16970 3603452..3603931 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
I6J91_RS16975 3604017..3604250 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
I6J91_RS16980 3604253..3604567 + 315 WP_001160966.1 CcdB family protein Toxin
I6J91_RS16985 3604564..3605412 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
I6J91_RS16990 3605419..3605796 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
I6J91_RS16995 3605799..3606620 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
I6J91_RS17000 3606617..3607606 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
I6J91_RS17005 3607606..3608892 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T190399 WP_001160966.1 NZ_CP069441:3604253-3604567 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T190399 NZ_CP091327:c54150-53734 [Klebsiella pneumoniae]
GTGAACAAAATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATTACCTACGCCGAGATGCGCTTCGGCGCCACCGGGC
CGAAGGCCGCGCCGCGTCACGTCCAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCATGGCCGGCACGCCGATTGGCCCGAACGACACGGCGATTGC
CGGACACGCCATCGCCGCCGGCGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT190399 WP_000125566.1 NZ_CP069441:3604017-3604250 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT190399 NZ_CP091327:c54377-54147 [Klebsiella pneumoniae]
ATGCGCACAGTCTCTATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References