Detailed information of TA system TA190396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3495538..3496046
• Replicon: chromosome
• Accession: NZ_CP069441
• Organism: Escherichia coli strain FDAARGOS_1261

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: I6J91_RS16445
• Protein ID: WP_001534843.1
• Coordinates: 3495538..3495747 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: I6J91_RS16450
• Protein ID: WP_000063161.1
• Coordinates: 3495744..3496046 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J91_RS16430	3491294..3492604	-	1311	WP_001534837.1	restriction endonuclease subunit S	-
I6J91_RS16435	3492594..3494212	-	1619	Protein_3205	type I restriction-modification system subunit M	-
I6J91_RS16440	3494276..3495442	-	1167	WP_001534841.1	restriction endonuclease	-
I6J91_RS16445	3495538..3495747	+	210	WP_001534843.1	BrnT family toxin	Toxin
I6J91_RS16450	3495744..3496046	+	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
I6J91_RS16455	3496080..3497036	-	957	WP_001534845.1	GTPase	-
I6J91_RS16460	3497047..3497250	-	204	WP_001534846.1	YbdD/YjiX family protein	-
I6J91_RS16465	3497368..3499518	-	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T190396 WP_001534843.1 NZ_CP069441:3495538-3495747 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T190396 NZ_CP091326:4273253-4273636 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCAGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT190396 WP_000063161.1 NZ_CP069441:3495744-3496046 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT190396 NZ_CP091326:4273011-4273253 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3