Detailed information of TA system TA190395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3452381..3453201
• Replicon: chromosome
• Accession: NZ_CP069441
• Organism: Escherichia coli strain FDAARGOS_1261

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: I6J91_RS16245
• Protein ID: WP_001054376.1
• Coordinates: 3452944..3453201 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: I6J91_RS16240
• Protein ID: WP_001519303.1
• Coordinates: 3452381..3452932 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J91_RS16205	3448076..3449389	-	1314	WP_001519300.1	galactitol-specific PTS transporter subunit IIC	-
I6J91_RS16210	3449401..3449679	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
I6J91_RS16215	3449676..3450797	-	1122	WP_000010833.1	M42 family metallopeptidase	-
I6J91_RS16220	3451042..3451158	-	117	Protein_3162	VOC family protein	-
I6J91_RS16225	3451196..3451456	-	261	WP_000084876.1	hypothetical protein	-
I6J91_RS16230	3451583..3452131	-	549	WP_001519301.1	hypothetical protein	-
I6J91_RS16235	3452168..3452329	-	162	WP_001519302.1	hypothetical protein	-
I6J91_RS16240	3452381..3452932	-	552	WP_001519303.1	N-acetyltransferase	Antitoxin
I6J91_RS16245	3452944..3453201	-	258	WP_001054376.1	hypothetical protein	Toxin
I6J91_RS16250	3453578..3453877	-	300	WP_001519304.1	GNAT family N-acetyltransferase	-
I6J91_RS16255	3453940..3455181	+	1242	Protein_3169	DNA helicase	-
I6J91_RS16260	3455764..3456744	-	981	WP_001519307.1	transposase	-
I6J91_RS16265	3456809..3457915	-	1107	WP_001519308.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	3452168..3464185	12017

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T190395 WP_001054376.1 NZ_CP069441:c3453201-3452944 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T190395 NZ_CP091326:c3994324-3993839 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20463.61 Da
• Isoelectric Point: 6.8758

>AT190395 WP_001519303.1 NZ_CP069441:c3452932-3452381 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT190395 NZ_CP091326:c3994578-3994312 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: S1PA82