Detailed information of TA system TA190387

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 574525..575356
• Replicon: chromosome
• Accession: NZ_CP069441
• Organism: Escherichia coli strain FDAARGOS_1261

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: I6J91_RS02720
• Protein ID: WP_000854814.1
• Coordinates: 574982..575356 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A366YXM1
• Locus tag: I6J91_RS02715
• Protein ID: WP_001518724.1
• Coordinates: 574525..574893 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J91_RS02685	570818..571471	+	654	WP_001535423.1	hypothetical protein	-
I6J91_RS02690	571487..571897	+	411	WP_000846705.1	hypothetical protein	-
I6J91_RS02695	572118..572939	+	822	WP_001234710.1	DUF945 domain-containing protein	-
I6J91_RS02700	573202..573675	+	474	WP_000855075.1	antirestriction protein	-
I6J91_RS02705	573691..574167	+	477	WP_001186773.1	RadC family protein	-
I6J91_RS02710	574230..574451	+	222	WP_000692323.1	DUF987 domain-containing protein	-
I6J91_RS02715	574525..574893	+	369	WP_001518724.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6J91_RS02720	574982..575356	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
I6J91_RS02725	575353..575547	+	195	WP_000988601.1	hypothetical protein	-
I6J91_RS02730	575560..575673	+	114	WP_001161660.1	DUF957 domain-containing protein	-
I6J91_RS02735	575962..576108	-	147	Protein_535	transposase domain-containing protein	-
I6J91_RS02740	576162..576344	+	183	WP_001016348.1	hypothetical protein	-
I6J91_RS02745	576445..576774	-	330	WP_000450409.1	DUF496 family protein	-
I6J91_RS02750	576946..578004	-	1059	WP_001200894.1	FUSC family protein	-
I6J91_RS02755	578202..578675	-	474	WP_001105376.1	DNA gyrase inhibitor SbmC	-
I6J91_RS02760	578794..579960	-	1167	WP_001297905.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T190387 WP_000854814.1 NZ_CP069441:574982-575356 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T190387 NZ_CP091321:c7699-7394 [Enterobacter sp. JH25]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13441.24 Da
• Isoelectric Point: 4.7594

>AT190387 WP_001518724.1 NZ_CP069441:574525-574893 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLSSDADAYPLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLACEADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT190387 NZ_CP091321:c7919-7701 [Enterobacter sp. JH25]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A366YXM1