Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 148775..149310 Replicon plasmid unnamed
Accession NZ_CP069439
Organism Escherichia coli strain FDAARGOS_1260

Toxin (Protein)


Gene name relE Uniprot ID A0A0M1UJC2
Locus tag I6J90_RS25130 Protein ID WP_001270422.1
Coordinates 149023..149310 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag I6J90_RS25125 Protein ID WP_001132895.1
Coordinates 148775..149026 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J90_RS25095 143792..144103 - 312 WP_001309256.1 hypothetical protein -
I6J90_RS25100 144167..144526 - 360 WP_001336695.1 DUF1281 domain-containing protein -
I6J90_RS25105 144533..144676 - 144 WP_000270807.1 DUF1281 domain-containing protein -
I6J90_RS25110 144843..145619 - 777 Protein_171 hypothetical protein -
I6J90_RS25115 145853..146815 - 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
I6J90_RS25120 146812..148017 - 1206 WP_000817635.1 AAA family ATPase -
I6J90_RS25125 148775..149026 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I6J90_RS25130 149023..149310 + 288 WP_001270422.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6J90_RS25135 149312..149723 + 412 Protein_176 DDE-type integrase/transposase/recombinase -
I6J90_RS25140 150367..151344 + 978 WP_001309253.1 RepB family plasmid replication initiator protein -
I6J90_RS25145 151587..152327 - 741 WP_001066947.1 tyrosine-type recombinase/integrase -
I6J90_RS25150 152448..152636 - 189 WP_001332784.1 hypothetical protein -
I6J90_RS25155 153003..154172 + 1170 WP_001072358.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 / mph(A) senB 1..168315 168315
- flank IS/Tn - - 145056..145196 140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11262.30 Da        Isoelectric Point: 10.4703

>T190378 WP_001270422.1 NZ_CP069439:149023-149310 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T190378 NZ_CP091319:c1061678-1061460 [Enterobacter sp. JH25]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT190378 WP_001132895.1 NZ_CP069439:148775-149026 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT190378 NZ_CP091319:c1062081-1061707 [Enterobacter sp. JH25]
TTGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M1UJC2

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References