Detailed information of TA system TA190364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3731789..3732297
• Replicon: chromosome
• Accession: NZ_CP069438
• Organism: Escherichia coli strain FDAARGOS_1260

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: I6J90_RS18140
• Protein ID: WP_001534843.1
• Coordinates: 3731789..3731998 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0V9L4L3
• Locus tag: I6J90_RS18145
• Protein ID: WP_000063161.1
• Coordinates: 3731995..3732297 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J90_RS18125	3727544..3728854	-	1311	WP_001534837.1	restriction endonuclease subunit S	-
I6J90_RS18130	3728844..3730463	-	1620	WP_001550561.1	type I restriction-modification system subunit M	-
I6J90_RS18135	3730527..3731693	-	1167	WP_001534841.1	restriction endonuclease	-
I6J90_RS18140	3731789..3731998	+	210	WP_001534843.1	BrnT family toxin	Toxin
I6J90_RS18145	3731995..3732297	+	303	WP_000063161.1	BrnA antitoxin family protein	Antitoxin
I6J90_RS18150	3732331..3733287	-	957	WP_001534845.1	GTPase	-
I6J90_RS18155	3733298..3733501	-	204	WP_001534846.1	YbdD/YjiX family protein	-
I6J90_RS18160	3733619..3735769	-	2151	WP_001366791.1	pyruvate/proton symporter BtsT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7952.17 Da
• Isoelectric Point: 4.9493

>T190364 WP_001534843.1 NZ_CP069438:3731789-3731998 [Escherichia coli]
MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKSNRVKHGIRFEDAVLVLDDPQRTEPL

Nucleotide

• Length: 210 bp

>T190364 NZ_CP091308:c1399576-1399235 [Burkholderia pyrrocinia]
ATGCGAACCGTCGCGGACGAAATCCATTTCTTCAGCGAGCAAGTCGAGGCTGGCGTATTTGCGTTGCCGAAAACGCTGAC
CGCCAGATTCTTCGCACTCGCTGACCGCATGGAGCGGTACGGCCCGAATCTGGGCGAACCTCACACCAGGCCGATGGGCG
ACGGGTTGTACGAAATGCGGTTGAAGGGCCTGGAAGGTATCGCGCGTGTGTTTTACTGCGCGGTCGTCAACCGGCGGATC
GTCATGCTTCACTGCTTCGTGAAGAAGACACGGAAAACGCCGCAGAAAGAGCTGGAAACCGCGCGCCGCCGATTGAAGGA
GATTCGAGATGGCAACCTATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11600.10 Da
• Isoelectric Point: 9.4646

>AT190364 WP_000063161.1 NZ_CP069438:3731995-3732297 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKVLANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT190364 NZ_CP091308:c1399248-1398961 [Burkholderia pyrrocinia]
ATGGCAACCTATAAAGCACTGCGCGAGCGCGCGTTGGCCGATCCGACGGTACGCGCCGAATACGAACGATTGAACCGCGA
GGAGTTCGCGCTGCTCGATGCCATGCTGGCCGCCCGGCGAGCCGCCGGTCTTTCGCAAGCCGATGTGGCAGAGCGCATGG
GCACGAAGGCGCCTGCCGTCACGCGGCTCGAACGCGCGCTCGCGACAGGCCAGCACTCTCCGTCGATTGAAACGCTGCGC
AAGTATGCGGCCGCGTGCGGCAAGAAACTCGTGATCTCGTTCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0V9L4L3