Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3407773..3408270 Replicon chromosome
Accession NZ_CP069438
Organism Escherichia coli strain FDAARGOS_1260

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag I6J90_RS16500 Protein ID WP_001173343.1
Coordinates 3408097..3408270 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag I6J90_RS16495 Protein ID WP_001312331.1
Coordinates 3407773..3408069 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6J90_RS16475 3404502..3405221 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6J90_RS16480 3405218..3406849 - 1632 WP_001216466.1 sensor histidine kinase -
I6J90_RS16485 3407030..3407260 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
I6J90_RS16490 3407272..3407544 + 273 WP_000405647.1 N-acetyltransferase -
I6J90_RS16495 3407773..3408069 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6J90_RS16500 3408097..3408270 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
I6J90_RS16505 3408389..3409906 - 1518 WP_000003805.1 lysine--tRNA ligase -
I6J90_RS16510 3410143..3411600 - 1458 WP_000856828.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T190361 WP_001173343.1 NZ_CP069438:3408097-3408270 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T190361 NZ_CP091308:300158-300424 [Burkholderia pyrrocinia]
ATGAGCGTACGCCGCGTGCTCGTTACTTCCGACGCGCGGGACGACTATGTCTATTGGCAAGGGCAGGATCGCAAGACGCT
CAAGCGCATCAACCAGCTCATTCGCGAAGCCCAGCGAACGCCGTTCGAAGGCATCGGCAAACCCGAACCTTTGAAGGCCC
GTCTCTCCGGCTTCTGGTCGCGCCGTATCGACGACACGAACCGCCCGGTCTACGAGGCTGACGACCTTCAGATCAGCATC
GTGTCCTGCCGCTATCACTACGAGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT190361 WP_001312331.1 NZ_CP069438:3407773-3408069 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT190361 NZ_CP091308:299895-300161 [Burkholderia pyrrocinia]
ATGCGCACGATTCATTTTTCGGATGCCCGCGGTAATCTGAAGACCGTCATCGACCAGGTTGTCGACGATGCGGACGTGAC
GCTCATCACGCGCCGCGATGCACCGAACGCCGTCATCATGTCGCAGGACTACTACGACAGCCTGATGGAGACGGTTCACT
TGCTGCGCTCGCCGGCCAACGTCGCCCATCTCGAACGTTCGATCGCGCAACTGCGCAAGGGCAAGGGCAAGGCGAAAGAA
CACAAGCTCGCCGAGGATGATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References