Detailed information of TA system TA190360

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2845907..2846742
• Replicon: chromosome
• Accession: NZ_CP069438
• Organism: Escherichia coli strain FDAARGOS_1260

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A4S3WCD6
• Locus tag: I6J90_RS13905
• Protein ID: WP_021566610.1
• Coordinates: 2846365..2846742 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A2J4HRK6
• Locus tag: I6J90_RS13900
• Protein ID: WP_001354276.1
• Coordinates: 2845907..2846275 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J90_RS13865	2841132..2842745	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
I6J90_RS13870	2842780..2842932	+	153	Protein_2719	DUF905 family protein	-
I6J90_RS13875	2843023..2843841	+	819	WP_001234748.1	DUF945 domain-containing protein	-
I6J90_RS13880	2843932..2844417	+	486	WP_000214318.1	antirestriction protein	-
I6J90_RS13885	2844432..2844908	+	477	WP_001186742.1	RadC family protein	-
I6J90_RS13890	2844977..2845198	+	222	WP_021566608.1	DUF987 domain-containing protein	-
I6J90_RS13895	2845213..2845857	+	645	Protein_2724	antitoxin of toxin-antitoxin stability system	-
I6J90_RS13900	2845907..2846275	+	369	WP_001354276.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6J90_RS13905	2846365..2846742	+	378	WP_021566610.1	TA system toxin CbtA family protein	Toxin
I6J90_RS13910	2846739..2847227	+	489	WP_048237286.1	hypothetical protein	-
I6J90_RS13915	2847239..2847436	+	198	WP_000839269.1	DUF957 domain-containing protein	-
I6J90_RS13920	2847521..2848366	+	846	WP_021566612.1	DUF4942 domain-containing protein	-
I6J90_RS13925	2848958..2849455	+	498	WP_000509810.1	hypothetical protein	-
I6J90_RS13930	2849622..2850545	+	924	WP_001445306.1	carboxylate/amino acid/amine transporter	-
I6J90_RS13935	2850549..2851367	-	819	WP_000779409.1	lipoprotein NlpA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.02 Da
• Isoelectric Point: 7.8276

>T190360 WP_021566610.1 NZ_CP069438:2846365-2846742 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T190360 NZ_CP091308:299895-300161 [Burkholderia pyrrocinia]
ATGCGCACGATTCATTTTTCGGATGCCCGCGGTAATCTGAAGACCGTCATCGACCAGGTTGTCGACGATGCGGACGTGAC
GCTCATCACGCGCCGCGATGCACCGAACGCCGTCATCATGTCGCAGGACTACTACGACAGCCTGATGGAGACGGTTCACT
TGCTGCGCTCGCCGGCCAACGTCGCCCATCTCGAACGTTCGATCGCGCAACTGCGCAAGGGCAAGGGCAAGGCGAAAGAA
CACAAGCTCGCCGAGGATGATGAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13649.47 Da
• Isoelectric Point: 7.0261

>AT190360 WP_001354276.1 NZ_CP069438:2845907-2846275 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPKMKN

Nucleotide

• Length: 369 bp

>AT190360 NZ_CP091308:300158-300424 [Burkholderia pyrrocinia]
ATGAGCGTACGCCGCGTGCTCGTTACTTCCGACGCGCGGGACGACTATGTCTATTGGCAAGGGCAGGATCGCAAGACGCT
CAAGCGCATCAACCAGCTCATTCGCGAAGCCCAGCGAACGCCGTTCGAAGGCATCGGCAAACCCGAACCTTTGAAGGCCC
GTCTCTCCGGCTTCTGGTCGCGCCGTATCGACGACACGAACCGCCCGGTCTACGAGGCTGACGACCTTCAGATCAGCATC
GTGTCCTGCCGCTATCACTACGAGTAG

Structures

Toxin

• AlphaFold DB: A0A4S3WCD6

Antitoxin

• AlphaFold DB: A0A2J4HRK6