Detailed information of TA system TA190356

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2004158..2004992
• Replicon: chromosome
• Accession: NZ_CP069438
• Organism: Escherichia coli strain FDAARGOS_1260

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: I6J90_RS09865
• Protein ID: WP_000854689.1
• Coordinates: 2004615..2004992 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1X3JHN3
• Locus tag: I6J90_RS09860
• Protein ID: WP_001285598.1
• Coordinates: 2004158..2004538 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J90_RS09825	2000441..2000896	+	456	WP_000581502.1	hypothetical protein	-
I6J90_RS09830	2001001..2001210	+	210	WP_032142237.1	DUF905 family protein	-
I6J90_RS09835	2001311..2002129	+	819	WP_001234620.1	DUF945 domain-containing protein	-
I6J90_RS09840	2002184..2002669	+	486	WP_059342223.1	antirestriction protein	-
I6J90_RS09845	2002685..2003161	+	477	WP_001186726.1	RadC family protein	-
I6J90_RS09850	2003224..2003445	+	222	WP_000692329.1	DUF987 domain-containing protein	-
I6J90_RS09855	2003464..2004108	+	645	WP_000094916.1	hypothetical protein	-
I6J90_RS09860	2004158..2004538	+	381	WP_001285598.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6J90_RS09865	2004615..2004992	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
I6J90_RS09870	2004989..2005476	+	488	Protein_1932	hypothetical protein	-
I6J90_RS09875	2005493..2005690	+	198	WP_000839293.1	DUF957 domain-containing protein	-
I6J90_RS09880	2005775..2006620	+	846	WP_023281696.1	DUF4942 domain-containing protein	-
I6J90_RS09885	2006689..2007084	+	396	WP_000208383.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
I6J90_RS09890	2007077..2007970	+	894	WP_059342195.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
I6J90_RS09895	2008441..2008611	+	171	Protein_1937	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T190356 WP_000854689.1 NZ_CP069438:2004615-2004992 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T190356 NZ_CP091307:c28540-28124 [Burkholderia pyrrocinia]
GTGACCACGCTCTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGGCCGCCCGTGGTGCTGTCGCGGTT
GCAGTCATGCGTCAACGCTCAGCACCGGATCGTCGTGTCGGCAATCACCTATGCCGAAATGCGATTCGGCGCGGTCGGCA
GGAAAGCGTCGCCGAAGCATGCGGAACTCGTGACGGCGTTTGTTTCGCGGCTGGATGGCGTGCTGTCGTGGGATACGGCG
GCCGTCGACGCAACGGCCGCGATTCGTGCGGATCTTGCCGCGCGCGGCATGCCGATCGGCGCGAACGATGCGTCGATCGC
AGGGCATGCGATCGCGGCCGGCGCCGTGCTCGTCACGAACAATGGCCGCGAATTCGGCCGTGTCGTAGGGTTGTCGCTCG
AAGACTGGGCGGCCTGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13927.72 Da
• Isoelectric Point: 4.7959

>AT190356 WP_001285598.1 NZ_CP069438:2004158-2004538 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT190356 NZ_CP091307:c28800-28537 [Burkholderia pyrrocinia]
ATGCGCACCGTTTCCATTTTCAAGAACGCCCGTAACCAGGCAATCCGCATCCCGAAGGACATGGAGTTCGAGGGGGTGAC
GGAACTCGAGATCCGCCGCGAGGGCGACACGCTGCTGCTGCGCCCCGTGCGCCCCACGTGGCTGTCGTTCGCGAACGAAC
CGCTGGCCGACGCCGAGTTCCTCGCCGAACGCCCGGCCGTCATCGAGTCCGGCCGTTTCGACCTGTCCGGCGACACCGGC
GCGCCGACGGAGTCCGGCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1X3JHN3