Detailed information of TA system TA190337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1430280..1431197
• Replicon: chromosome
• Accession: NZ_CP069391
• Organism: Bacillus velezensis strain Sam8H1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6A8LGT8
• Locus tag: JR311_RS07425
• Protein ID: WP_003154806.1
• Coordinates: 1430451..1431197 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: JR311_RS07420
• Protein ID: WP_003154807.1
• Coordinates: 1430280..1430450 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR311_RS07385	1425492..1427081	+	1590	WP_156735151.1	hypothetical protein	-
JR311_RS07390	1427094..1427519	+	426	WP_156735150.1	hypothetical protein	-
JR311_RS07395	1427524..1427721	+	198	WP_003154819.1	XkdX family protein	-
JR311_RS07400	1427778..1428539	+	762	WP_204530484.1	phage portal protein	-
JR311_RS07405	1428591..1428854	+	264	WP_003154815.1	hemolysin XhlA family protein	-
JR311_RS07410	1428868..1429131	+	264	WP_204530485.1	phage holin	-
JR311_RS07415	1429145..1430023	+	879	WP_109566884.1	N-acetylmuramoyl-L-alanine amidase	-
JR311_RS07420	1430280..1430450	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
JR311_RS07425	1430451..1431197	-	747	WP_003154806.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JR311_RS07430	1431302..1432300	-	999	WP_003154805.1	inorganic phosphate transporter	-
JR311_RS07435	1432313..1432930	-	618	WP_003154804.1	DUF47 domain-containing protein	-
JR311_RS07440	1433216..1434532	-	1317	WP_165408947.1	amino acid permease	-
JR311_RS07445	1434854..1435804	+	951	WP_077722102.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29034.50 Da
• Isoelectric Point: 4.6947

>T190337 WP_003154806.1 NZ_CP069391:c1431197-1430451 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T190337 NZ_CP091299:c5385-4969 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATCGTGGTCTCGGCCATCACTTACTCCGAGATGCGCTTCGGTGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCGTCCTGCCCTGGGACCGTGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTTCGCATGGCCGGCACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGCGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCGGGACTGGTACTCG
AAGACTGGGTAAAATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT190337 WP_003154807.1 NZ_CP069391:c1430450-1430280 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT190337 NZ_CP091299:c5612-5382 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGTGACATGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTCG
AAAAGGCAGATCCGGACTTTATGGCGGAGCGCGAAGACGTTGTCACCGATGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A6A8LGT8

Antitoxin

• AlphaFold DB: I2HQ14