Detailed information of TA system TA190336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 702944..703581
• Replicon: chromosome
• Accession: NZ_CP069391
• Organism: Bacillus velezensis strain Sam8H1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: JR311_RS03565
• Protein ID: WP_003156187.1
• Coordinates: 703231..703581 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: JR311_RS03560
• Protein ID: WP_003156188.1
• Coordinates: 702944..703225 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JR311_RS03540	699309..699908	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
JR311_RS03545	700001..700366	+	366	WP_070081400.1	holo-ACP synthase	-
JR311_RS03550	700531..701538	+	1008	WP_204530297.1	outer membrane lipoprotein carrier protein LolA	-
JR311_RS03555	701655..702824	+	1170	WP_198863500.1	alanine racemase	-
JR311_RS03560	702944..703225	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
JR311_RS03565	703231..703581	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
JR311_RS03570	703699..704520	+	822	WP_003156182.1	STAS domain-containing protein	-
JR311_RS03575	704525..704890	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
JR311_RS03580	704893..705294	+	402	WP_003156178.1	anti-sigma regulatory factor	-
JR311_RS03585	705306..706313	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
JR311_RS03590	706377..706706	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
JR311_RS03595	706703..707185	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
JR311_RS03600	707151..707939	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
JR311_RS03605	707939..708541	+	603	WP_204530298.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T190336 WP_003156187.1 NZ_CP069391:703231-703581 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T190336 NZ_CP091296:c21962-21618 [Lactiplantibacillus plantarum subsp. plantarum]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTGGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT190336 WP_003156188.1 NZ_CP069391:702944-703225 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT190336 NZ_CP091296:c22219-21956 [Lactiplantibacillus plantarum subsp. plantarum]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5