Detailed information of TA system TA190334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 846939..847478
• Replicon: chromosome
• Accession: NZ_CP069389
• Organism: Mammaliicoccus sciuri strain GDQ20D70P

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A8E2VFW5
• Locus tag: JRU67_RS04200
• Protein ID: WP_025904511.1
• Coordinates: 847107..847478 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A657XHG2
• Locus tag: JRU67_RS04195
• Protein ID: WP_025904510.1
• Coordinates: 846939..847106 (+)
• Length: 56 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRU67_RS04170	842822..843301	+	480	WP_159315401.1	PH domain-containing protein	-
JRU67_RS04175	843291..844811	+	1521	WP_198471926.1	PH domain-containing protein	-
JRU67_RS04180	844801..845265	+	465	WP_159315399.1	PH domain-containing protein	-
JRU67_RS04185	845296..845658	+	363	WP_198472315.1	holo-ACP synthase	-
JRU67_RS04190	845706..846854	+	1149	WP_159315398.1	alanine racemase	-
JRU67_RS04195	846939..847106	+	168	WP_025904510.1	hypothetical protein	Antitoxin
JRU67_RS04200	847107..847478	+	372	WP_025904511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JRU67_RS04205	847575..848579	+	1005	WP_037589756.1	PP2C family protein-serine/threonine phosphatase	-
JRU67_RS04210	848684..849007	+	324	WP_025904513.1	anti-sigma factor antagonist	-
JRU67_RS04215	849012..849488	+	477	WP_025904514.1	anti-sigma B factor RsbW	-
JRU67_RS04220	849463..850233	+	771	WP_037589755.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13641.78 Da
• Isoelectric Point: 9.9193

>T190334 WP_025904511.1 NZ_CP069389:847107-847478 [Mammaliicoccus sciuri]
MRRGDVYLADLSPVTGSEQGGTRPVVIIQNDTGNRYSPTVIVAAITGKINKAKIPTHVEIEAAKYKLDRDSVILLEQIRT
IDKKRLKEKLTYLSDAKMKEVDTAIAISLNLTLQQKFDTLGNT

Nucleotide

• Length: 372 bp

>T190334 NZ_CP091289:1263991-1264293 [Lacticaseibacillus paracasei subsp. paracasei]
GTGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin

Protein

• Length: 56 a.a.
• Molecular weight: 6136.65 Da
• Isoelectric Point: 4.0121

>AT190334 WP_025904510.1 NZ_CP069389:846939-847106 [Mammaliicoccus sciuri]
MTSLTQNRTQSLEQTLKEGYRSMADLNLSLASEAYYCECEACDSNESHIASQQGE

Nucleotide

• Length: 168 bp

>AT190334 NZ_CP091289:1263729-1263998 [Lacticaseibacillus paracasei subsp. paracasei]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAGGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACACCT
GATGACATGTGGAGAGACTTGAGTGTATAG

Structures

Antitoxin

• AlphaFold DB: A0A657XHG2