Detailed information of TA system TA190333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 97875..98983
• Replicon: chromosome
• Accession: NZ_CP069389
• Organism: Mammaliicoccus sciuri strain GDQ20D70P

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: JRU67_RS00440
• Protein ID: WP_000233000.1
• Coordinates: 97875..98744 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: JRU67_RS00445
• Protein ID: WP_000205227.1
• Coordinates: 98759..98983 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JRU67_RS00410	93452..94186	-	735	WP_044291471.1	glucose 1-dehydrogenase	-
JRU67_RS00415	94274..95338	-	1065	Protein_80	recombinase family protein	-
JRU67_RS00420	95413..96093	+	681	WP_044291606.1	IS6-like element IS1216 family transposase	-
JRU67_RS00425	96149..96220	-	72	Protein_82	adenine phosphoribosyltransferase	-
JRU67_RS00430	96264..97127	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
JRU67_RS00435	97160..97894	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
JRU67_RS00440	97875..98744	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
JRU67_RS00445	98759..98983	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
JRU67_RS00450	99126..100670	-	1545	WP_002390960.1	recombinase family protein	-
JRU67_RS00455	100690..101106	-	417	WP_000323438.1	recombinase	-
JRU67_RS00460	101107..101403	-	297	WP_002360685.1	hypothetical protein	-
JRU67_RS00465	101951..103114	-	1164	WP_025905227.1	chromate efflux transporter	-
JRU67_RS00470	103125..103427	-	303	WP_025905228.1	helix-turn-helix transcriptional regulator	-
JRU67_RS00475	103553..103954	-	402	WP_025905229.1	arsenate reductase (thioredoxin)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	ant(6)-Ia	-	95413..97127	1714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T190333 WP_000233000.1 NZ_CP069389:c98744-97875 [Mammaliicoccus sciuri]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T190333 NZ_CP091289:c447708-447340 [Lacticaseibacillus paracasei subsp. paracasei]
ATGTATAAAATTGACTTCTATGAGGATCGCGATGGCTACTCTGAGATCGAAGACTTTCTTGATCAGTTACGCCATAGTCA
TCAAAAAAATAATGTGAGCCTTTTAAACAAAATCACAAAGGAACTCTACATGCTGCAAAATCTTGGTCCGCGCTTACGTG
AACCTCACGCGAAGTTTCTGAAGGGATATGCTTATCCGATCATGGAATTGCGTCCCATGCCAGAAAGAATCTTTTATGCT
GCATGGCAAAAGGATCATTTCGTTTTGCTTCATCACTACACCAAGAAAACAAACAAAACTGATCACAGAGAAATTTTACG
GGCACAGGCTAACCTCGAAGATTGGCTCTCTAGAAAGGAAGAAAATTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT190333 WP_000205227.1 NZ_CP069389:c98983-98759 [Mammaliicoccus sciuri]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT190333 NZ_CP091289:c447340-447080 [Lacticaseibacillus paracasei subsp. paracasei]
ATGACGACATGGGAAGACTTCAGTCAGAAACTGAAGAAGGACAATCCAGAAAAGTTGGCAATCATCAAAACCTTGGCCGA
TTTAGAAAGTGAACGAATCAAGCAAGGAATCTCTCAAAAGGACTTTGCAGAAAAAATTGGTATGAAACAGCCGCAACTAG
CAAAGATTGAGCGCTTAGACTCTCTTCCGACTCTGGCAACACTCAATCGATACGCGAGAGGGCTTGGAAAAGAAATTCGT
TTATCAATTGTTACTCAGTGA

Structures

Toxin

• AlphaFold DB: R3JIN1