Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3865281..3865899 Replicon chromosome
Accession NZ_CP069386
Organism Escherichia coli strain XH1815

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JSW75_RS18725 Protein ID WP_001291435.1
Coordinates 3865281..3865499 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JSW75_RS18730 Protein ID WP_000344800.1
Coordinates 3865525..3865899 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSW75_RS18690 (3860570) 3860570..3861142 + 573 WP_000779842.1 YbaY family lipoprotein -
JSW75_RS18695 (3861173) 3861173..3861484 - 312 WP_000409911.1 MGMT family protein -
JSW75_RS18705 (3861863) 3861863..3862216 + 354 WP_000878140.1 DUF1428 domain-containing protein -
JSW75_RS18710 (3862258) 3862258..3863808 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JSW75_RS18715 (3863972) 3863972..3864442 - 471 WP_001716237.1 YlaC family protein -
JSW75_RS18720 (3864558) 3864558..3865109 - 552 WP_000102564.1 maltose O-acetyltransferase -
JSW75_RS18725 (3865281) 3865281..3865499 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JSW75_RS18730 (3865525) 3865525..3865899 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JSW75_RS18735 (3866445) 3866445..3869594 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JSW75_RS18740 (3869617) 3869617..3870810 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T190326 WP_001291435.1 NZ_CP069386:c3865499-3865281 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T190326 NZ_CP091245:15476-15778 [Thiothrix winogradskyi]
ATGCCATCATTAATTTTTAGCCATACCGCCCTACTGGACTTAGAGCGGTTACGGGCATTCTTACGCGCCAAAAACCGACC
AGCCGCCCGCCGTGCCGTGGTCAAGATCATGCAAGGGTTGCAGGCACTGGAACAGCACCCGGAGCTAGGTCACACGGTGG
AAGACCGACCGGAAGACTACCGCGAGCTAGTGATTCCCTTCGGCAAAGACGGCTACATTGCCGCCTACCGTTACGCGGGG
GATTCGCTGGTGGTGTTGGGTGTCCGCCATCAACGCGAGTTACAGCCGCCTACCCTGCCCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT190326 WP_000344800.1 NZ_CP069386:c3865899-3865525 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT190326 NZ_CP091245:15195-15488 [Thiothrix winogradskyi]
ATGTCACAAGTTGTTTCCATTGCACCTACCTCTATCAAACTGGACGTAGACACCAAAACTCGCCTGCAACGCTTGGCAAC
CTCGCGCCAGCGTTCCACCCATTGGCTGATGCAAGAAGCCATCCGCCAGTATTTGGAGCGTGAGGAACAATTGGCGCAGT
TCCGCGACGAAATGCAAACCGCGTGGGATGATTACCAAGACACTGGCTTACACGTCACTGGCAAAGAAGTGTTTGCTTGG
ATGGAAACATGGTTCACTGATAGCGAAGCCCAGCCCCCCAAATGCCATCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References