Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3741541..3742220 | Replicon | chromosome |
| Accession | NZ_CP069386 | ||
| Organism | Escherichia coli strain XH1815 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | JSW75_RS18115 | Protein ID | WP_000854672.1 |
| Coordinates | 3741879..3742220 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | JSW75_RS18110 | Protein ID | WP_000070395.1 |
| Coordinates | 3741541..3741858 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JSW75_RS18060 (3736588) | 3736588..3737451 | + | 864 | WP_001065553.1 | GTPase family protein | - |
| JSW75_RS18065 (3737543) | 3737543..3738364 | + | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| JSW75_RS18070 (3738581) | 3738581..3738772 | + | 192 | Protein_3526 | DeoR family transcriptional regulator | - |
| JSW75_RS18075 (3738819) | 3738819..3738995 | + | 177 | WP_001285112.1 | hypothetical protein | - |
| JSW75_RS18080 (3738995) | 3738995..3739438 | + | 444 | WP_000824223.1 | lipoprotein YafY | - |
| JSW75_RS18085 (3739461) | 3739461..3739928 | + | 468 | WP_001547765.1 | protein YkfB | - |
| JSW75_RS18090 (3740005) | 3740005..3740244 | + | 240 | WP_000194654.1 | DUF905 family protein | - |
| JSW75_RS18095 (3740342) | 3740342..3740800 | + | 459 | WP_000211838.1 | antirestriction protein | - |
| JSW75_RS18100 (3740816) | 3740816..3741292 | + | 477 | WP_000811693.1 | RadC family protein | - |
| JSW75_RS18105 (3741301) | 3741301..3741522 | + | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| JSW75_RS18110 (3741541) | 3741541..3741858 | + | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| JSW75_RS18115 (3741879) | 3741879..3742220 | + | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| JSW75_RS18125 (3742792) | 3742792..3744045 | - | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| JSW75_RS18130 (3744057) | 3744057..3745160 | - | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| JSW75_RS18135 (3745448) | 3745448..3746503 | + | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| JSW75_RS18140 (3746542) | 3746542..3746634 | - | 93 | Protein_3539 | sigma factor-binding protein Crl | - |
| JSW75_RS18145 (3747086) | 3747086..3747087 | - | 2 | WP_001743396.1 | IS1-like element IS1A family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3722362..3747339 | 24977 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T190325 WP_000854672.1 NZ_CP069386:3741879-3742220 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T190325 NZ_CP091244:c3160176-3159991 [Thiothrix winogradskyi]
ATGAATAGCCGTGAACTGATAAAACTACTGAAAGCACACGGCTGGGAACTGGCACGAACGCGGGGCAGTCACCACCAATT
CAAGCACCCAGACCACGGGCATACCGTCACCGTGCCACACCCAAAGAAAGATCTAGGGGCGGGCATTGTGAAAGCCATTA
AACGGCAAGCCGGAATAGAGGATTAA
ATGAATAGCCGTGAACTGATAAAACTACTGAAAGCACACGGCTGGGAACTGGCACGAACGCGGGGCAGTCACCACCAATT
CAAGCACCCAGACCACGGGCATACCGTCACCGTGCCACACCCAAAGAAAGATCTAGGGGCGGGCATTGTGAAAGCCATTA
AACGGCAAGCCGGAATAGAGGATTAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT190325 WP_000070395.1 NZ_CP069386:3741541-3741858 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT190325 NZ_CP091244:c3159987-3159580 [Thiothrix winogradskyi]
ATGTACTACCCAATAGCCATAGAGCAAGGCGACGCTAACCACGCTTACGGCGTGATTGTGCCAGACATTCCGGGGTGCTT
TTCAGCGGGGGATACCTTGGATGAAGCACTGATGCAGGCAGAGGATGCAATCCTATTGCAGCTTGAAGAGTATCTGGATA
ACGGGAAACCCTTCCCGCAACCCTCACCAATGGAAGCATTACGCCACCAGAAAGCCTATGAGGGGTTCATTTGGTCAATC
ACCAAGGTGGATGTGAGCAAACTATCAGGCAAGTCCACACGGGTGAATATCAGTCTGCCGGAACGTATCCTTCAACTCAT
AGACGCGGCGGCGGAACGTGACGGCGAAACCCGTTCAGGCTTCATTGCTCGTGCTGCACTAGGGCATATCCGGCAATCCG
CACACTGA
ATGTACTACCCAATAGCCATAGAGCAAGGCGACGCTAACCACGCTTACGGCGTGATTGTGCCAGACATTCCGGGGTGCTT
TTCAGCGGGGGATACCTTGGATGAAGCACTGATGCAGGCAGAGGATGCAATCCTATTGCAGCTTGAAGAGTATCTGGATA
ACGGGAAACCCTTCCCGCAACCCTCACCAATGGAAGCATTACGCCACCAGAAAGCCTATGAGGGGTTCATTTGGTCAATC
ACCAAGGTGGATGTGAGCAAACTATCAGGCAAGTCCACACGGGTGAATATCAGTCTGCCGGAACGTATCCTTCAACTCAT
AGACGCGGCGGCGGAACGTGACGGCGAAACCCGTTCAGGCTTCATTGCTCGTGCTGCACTAGGGCATATCCGGCAATCCG
CACACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|