Detailed information of TA system TA190317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3161252..3162066
• Replicon: chromosome
• Accession: NZ_CP069386
• Organism: Escherichia coli strain XH1815

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: JSW75_RS15205
• Protein ID: WP_001054376.1
• Coordinates: 3161809..3162066 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: JSW75_RS15200
• Protein ID: WP_001309181.1
• Coordinates: 3161252..3161797 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JSW75_RS15170 (3156943)	3156943..3158256	-	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-
JSW75_RS15175 (3158268)	3158268..3158546	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
JSW75_RS15180 (3158543)	3158543..3159664	-	1122	WP_000010829.1	M42 family metallopeptidase	-
JSW75_RS15185 (3159909)	3159909..3160025	-	117	Protein_2960	VOC family protein	-
JSW75_RS15190 (3160063)	3160063..3160281	-	219	Protein_2961	hypothetical protein	-
JSW75_RS15195 (3160450)	3160450..3161196	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
JSW75_RS15200 (3161252)	3161252..3161797	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
JSW75_RS15205 (3161809)	3161809..3162066	-	258	WP_001054376.1	YjhX family toxin	Toxin
JSW75_RS23205 (3162557)	3162557..3162688	-	132	WP_001309182.1	hypothetical protein	-
JSW75_RS15215 (3162804)	3162804..3164044	+	1241	Protein_2966	helicase YjhR	-
JSW75_RS23210 (3164312)	3164312..3164517	-	206	Protein_2967	HNH endonuclease	-
JSW75_RS15220 (3164627)	3164627..3165607	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JSW75_RS15225 (3165672)	3165672..3166778	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T190317 WP_001054376.1 NZ_CP069386:c3162066-3161809 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T190317 NZ_CP091240:2728131-2728274 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT190317 WP_001309181.1 NZ_CP069386:c3161797-3161252 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT190317 NZ_CP091240:c2728468-2728206 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8