Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2980600..2981097 Replicon chromosome
Accession NZ_CP069386
Organism Escherichia coli strain XH1815

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag JSW75_RS14280 Protein ID WP_001173343.1
Coordinates 2980924..2981097 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag JSW75_RS14275 Protein ID WP_000398619.1
Coordinates 2980600..2980896 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSW75_RS14255 (2977331) 2977331..2978050 - 720 WP_000611288.1 two-component system response regulator DcuR -
JSW75_RS14260 (2978047) 2978047..2979678 - 1632 WP_001216477.1 sensor histidine kinase -
JSW75_RS14265 (2979859) 2979859..2980089 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
JSW75_RS14270 (2980101) 2980101..2980373 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
JSW75_RS14275 (2980600) 2980600..2980896 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
JSW75_RS14280 (2980924) 2980924..2981097 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
JSW75_RS14285 (2981216) 2981216..2982733 - 1518 WP_001295074.1 lysine--tRNA ligase -
JSW75_RS14290 (2982970) 2982970..2984427 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T190315 WP_001173343.1 NZ_CP069386:2980924-2981097 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T190315 NZ_CP091240:2727760-2727900 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT190315 WP_000398619.1 NZ_CP069386:2980600-2980896 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT190315 NZ_CP091240:c2728034-2727833 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTGTAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References