Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1665197..1665851 Replicon chromosome
Accession NZ_CP069386
Organism Escherichia coli strain XH1815

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JSW75_RS08040 Protein ID WP_000244777.1
Coordinates 1665197..1665604 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JSW75_RS08045 Protein ID WP_000354046.1
Coordinates 1665585..1665851 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSW75_RS08020 (1661154) 1661154..1662887 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
JSW75_RS08025 (1662893) 1662893..1663603 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JSW75_RS08030 (1663628) 1663628..1664524 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JSW75_RS08035 (1664636) 1664636..1665157 + 522 WP_001055874.1 flavodoxin FldB -
JSW75_RS08040 (1665197) 1665197..1665604 - 408 WP_000244777.1 protein YgfX Toxin
JSW75_RS08045 (1665585) 1665585..1665851 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JSW75_RS08050 (1666094) 1666094..1667074 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JSW75_RS08055 (1667270) 1667270..1667929 - 660 WP_072647361.1 hemolysin III family protein -
JSW75_RS08060 (1668093) 1668093..1668404 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
JSW75_RS08065 (1668449) 1668449..1669882 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
JSW75_RS08070 (1669939) 1669939..1670682 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T190309 WP_000244777.1 NZ_CP069386:c1665604-1665197 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T190309 NZ_CP091240:c2632907-2632764 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTTCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT190309 WP_000354046.1 NZ_CP069386:c1665851-1665585 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT190309 NZ_CP091240:2632647-2632831 [Enterococcus faecalis]
TGCTAGAATGTAGATGAAAAGAGAGAGATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTGAGACGGTGACCAATTGT
ATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGAAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References