190306

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1390701..1392138 Replicon chromosome
Accession NZ_CP069386
Organism Escherichia coli strain XH1815

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag JSW75_RS06745 Protein ID WP_000155570.1
Coordinates 1390701..1391774 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag JSW75_RS06750 Protein ID WP_000461704.1
Coordinates 1391767..1392138 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSW75_RS06730 (1386134) 1386134..1388323 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
JSW75_RS06735 (1388320) 1388320..1389936 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
JSW75_RS06740 (1390296) 1390296..1390559 - 264 WP_000502050.1 DUF1524 domain-containing protein -
JSW75_RS06745 (1390701) 1390701..1391774 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
JSW75_RS06750 (1391767) 1391767..1392138 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
JSW75_RS06755 (1392493) 1392493..1393356 + 864 WP_000787597.1 GTPase family protein -
JSW75_RS06760 (1393448) 1393448..1394269 + 822 WP_000197391.1 DUF932 domain-containing protein -
JSW75_RS06765 (1394486) 1394486..1395187 + 702 WP_000189409.1 WYL domain-containing protein -
JSW75_RS06770 (1395228) 1395228..1395464 + 237 WP_001144029.1 protein YpjK -
JSW75_RS06775 (1395464) 1395464..1395907 + 444 WP_000824222.1 lipoprotein YfjS -
JSW75_RS06780 (1395931) 1395931..1396398 + 468 WP_000702495.1 protein YfjT -
JSW75_RS23165 (1396785) 1396785..1396937 - 153 Protein_1333 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T190306 WP_000155570.1 NZ_CP069386:1390701-1391774 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T190306 NZ_CP091238:c62930-62067 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT190306 WP_000461704.1 NZ_CP069386:1391767-1392138 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT190306 NZ_CP091238:c63204-62932 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References