Detailed information of TA system TA190265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 58761..59373
• Replicon: plasmid unnamed1
• Accession: NZ_CP069371
• Organism: Neotabrizicola shimadae strain N10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8G0ZZQ7
• Locus tag: JO391_RS20525
• Protein ID: WP_018065892.1
• Coordinates: 58761..59156 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1U9Z9W2
• Locus tag: JO391_RS20530
• Protein ID: WP_018065893.1
• Coordinates: 59146..59373 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JO391_RS20505 (JO391_20485)	55371..55967	+	597	WP_220664760.1	class I SAM-dependent methyltransferase	-
JO391_RS20510 (JO391_20490)	56024..56371	+	348	WP_220664761.1	rhodanese-like domain-containing protein	-
JO391_RS20515 (JO391_20495)	56498..57571	-	1074	WP_220664767.1	hypothetical protein	-
JO391_RS20520 (JO391_20500)	57798..58682	+	885	WP_018065891.1	recombinase family protein	-
JO391_RS20525 (JO391_20505)	58761..59156	-	396	WP_018065892.1	PIN domain-containing protein	Toxin
JO391_RS20530 (JO391_20510)	59146..59373	-	228	WP_018065893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JO391_RS20535 (JO391_20515)	59583..60776	+	1194	WP_220664762.1	plasmid partitioning protein RepA	-
JO391_RS20540 (JO391_20520)	60790..61692	+	903	WP_259444929.1	plasmid partitioning protein RepB	-
JO391_RS20545 (JO391_20525)	61905..63116	+	1212	WP_220664650.1	plasmid replication protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..119227	119227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14256.28 Da
• Isoelectric Point: 4.1653

>T190265 WP_018065892.1 NZ_CP069371:c59156-58761 [Neotabrizicola shimadae]
MSAEFADTNVVLYLLDDGPKADRAEIILGQGPRISVQVLNETLVNCRRKAGLSWEEAGAFLEGVRALCPVEDLTLQTHDV
GRALAERYGFSIYDAMIVASALVAGCTTLWSEDMQDGLLVEGQLRIINPFA

Nucleotide

• Length: 396 bp

>T190265 NZ_CP091217:475063-475404 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8517.77 Da
• Isoelectric Point: 10.7157

>AT190265 WP_018065893.1 NZ_CP069371:c59373-59146 [Neotabrizicola shimadae]
MQVAKWGNSLAVRLPAELVRELGLKEGDQIDLVKDDGQVRVRRLARADEVLTGLRRFRGKLPAAERLSRDDAHER

Nucleotide

• Length: 228 bp

>AT190265 NZ_CP091217:474833-475063 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A1U9Z9W2