Detailed information of TA system TA190264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2690281..2690722
• Replicon: chromosome
• Accession: NZ_CP069361
• Organism: Burkholderia pseudomallei strain VB29710

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: JQX71_RS29785
• Protein ID: WP_009939402.1
• Coordinates: 2690549..2690722 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: JQX71_RS29780
• Protein ID: WP_203438891.1
• Coordinates: 2690281..2690565 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQX71_RS29755	2685386..2686648	-	1263	WP_038765621.1	c-type cytochrome	-
JQX71_RS29760	2686807..2688270	-	1464	WP_203438890.1	metallophosphoesterase	-
JQX71_RS29765	2688517..2688633	+	117	Protein_2176	IS3 family transposase	-
JQX71_RS29770	2688860..2689288	-	429	Protein_2177	IS110 family transposase	-
JQX71_RS29775	2689251..2689634	-	384	Protein_2178	DDE-type integrase/transposase/recombinase	-
JQX71_RS29780	2690281..2690565	-	285	WP_203438891.1	BrnA antitoxin family protein	Antitoxin
JQX71_RS29785	2690549..2690722	-	174	WP_009939402.1	BrnT family toxin	Toxin
JQX71_RS29790	2691390..2691665	+	276	WP_004552388.1	bacteriophage protein Gp49	-
JQX71_RS29795	2691675..2691806	+	132	WP_009923452.1	bacteriophage protein Gp48	-
JQX71_RS29800	2691944..2692186	+	243	WP_004545584.1	hypothetical protein	-
JQX71_RS29805	2692183..2692341	+	159	WP_004549802.1	hypothetical protein	-
JQX71_RS29810	2692389..2692607	+	219	WP_004552392.1	hypothetical protein	-
JQX71_RS29815	2692793..2693446	-	654	WP_004545536.1	hypothetical protein	-
JQX71_RS29820	2693695..2693844	+	150	WP_004537646.1	bacteriophage protein Gp44	-
JQX71_RS29825	2693841..2694614	+	774	Protein_2188	prohibitin family protein	-
JQX71_RS29830	2694612..2695040	+	429	Protein_2189	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T190264 WP_009939402.1 NZ_CP069361:c2690722-2690549 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T190264 NZ_CP091217:185259-185525 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10685.05 Da
• Isoelectric Point: 5.8930

>AT190264 WP_203438891.1 NZ_CP069361:c2690565-2690281 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGTAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT190264 NZ_CP091217:184996-185259 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA