Detailed information of TA system TA190262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 431991..432612
• Replicon: plasmid pMVF16-5
• Accession: NZ_CP069359
• Organism: Microvirga sp. VF16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JO965_RS47500
• Protein ID: WP_203448244.1
• Coordinates: 431991..432392 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JO965_RS47505
• Protein ID: WP_203448246.1
• Coordinates: 432382..432612 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JO965_RS47480	427082..428290	-	1209	WP_203448236.1	plasmid partitioning protein RepA	-
JO965_RS47485	428758..429165	-	408	WP_203448238.1	mismatch-specific DNA-glycosylase	-
JO965_RS47490	429685..430497	+	813	WP_203448240.1	APH(6) family putative aminoglycoside O-phosphotransferase	-
JO965_RS47495	431016..431933	-	918	WP_203448242.1	recombinase family protein	-
JO965_RS47500	431991..432392	-	402	WP_203448244.1	PIN domain-containing protein	Toxin
JO965_RS47505	432382..432612	-	231	WP_203448246.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JO965_RS47510	433444..434391	+	948	WP_203448484.1	DUF1612 domain-containing protein	-
JO965_RS47515	434575..435189	+	615	WP_203448248.1	hypothetical protein	-
JO965_RS47520	435283..435870	+	588	WP_203448250.1	hypothetical protein	-
JO965_RS47525	436046..437548	-	1503	WP_203448252.1	amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..456043	456043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• SpoVT-AbrB domain (5-36)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14411.47 Da
• Isoelectric Point: 4.4493

>T190262 WP_203448244.1 NZ_CP069359:c432392-431991 [Microvirga sp. VF16]
MSGKAFLDTNVVLYLLSEDAAKADRAEALLASGGTVSVQVLNEAVSVMTRKLKMDLDEVREVLAGVRHFCAVEPITTDTH
DVALNVMKRYGFSIYDSLIVAAAVSAGCETLFSEDLQDGQRIQDSLTIVNPFK

Nucleotide

• Length: 402 bp

>T190262 NZ_CP091213:185934-186200 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATATTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGCGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8397.65 Da
• Isoelectric Point: 10.5847

>AT190262 WP_203448246.1 NZ_CP069359:c432612-432382 [Microvirga sp. VF16]
MQVAKWGNSLAVRLPSAVVDALKLKEGDDIEIVVAGSRSFEVSKAPDAAETLARLRRFRGRLPAGFKFDRLEANER

Nucleotide

• Length: 231 bp

>AT190262 NZ_CP091213:185671-185934 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA