Detailed information of TA system TA190261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 310613..311234
• Replicon: plasmid pMVF16-4
• Accession: NZ_CP069358
• Organism: Microvirga sp. VF16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JO965_RS44335
• Protein ID: WP_203447503.1
• Coordinates: 310833..311234 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JO965_RS44330
• Protein ID: WP_203447502.1
• Coordinates: 310613..310843 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JO965_RS44305	305952..307229	+	1278	WP_203447500.1	DUF2183 domain-containing protein	-
JO965_RS44310	307353..307925	+	573	Protein_279	L,D-transpeptidase	-
JO965_RS44315	308193..308507	+	315	WP_203447931.1	DUF1311 domain-containing protein	-
JO965_RS44320	308674..308964	-	291	WP_203447501.1	hypothetical protein	-
JO965_RS44325	309035..309979	-	945	WP_203447934.1	DUF1612 domain-containing protein	-
JO965_RS44330	310613..310843	+	231	WP_203447502.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JO965_RS44335	310833..311234	+	402	WP_203447503.1	PIN domain-containing protein	Toxin
JO965_RS44340	311289..312209	+	921	WP_203447504.1	recombinase family protein	-
JO965_RS44345	312431..312937	+	507	WP_203447505.1	hypothetical protein	-
JO965_RS44350	314506..315744	+	1239	WP_203447506.1	plasmid partitioning protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..550688	550688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• SpoVT-AbrB domain (5-32)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14577.73 Da
• Isoelectric Point: 4.6998

>T190261 WP_203447503.1 NZ_CP069358:310833-311234 [Microvirga sp. VF16]
MSAKPFLDTNVILYLLSEDTTKADRAEALLAQGGTVSVQVLNEAANVMTRKLKMDLDEVGDILAGVRHFCEVVPVTIEIH
DQALAITRRFGFSVYDSLIVAAAIDAKCEVLYSEDLQHGQQVGARLTITNPFR

Nucleotide

• Length: 402 bp

>T190261 NZ_CP091210:467280-467621 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8386.64 Da
• Isoelectric Point: 8.8337

>AT190261 WP_203447502.1 NZ_CP069358:310613-310843 [Microvirga sp. VF16]
MQVSKWGNSLAVRLPAAVVEALDLKEGDDIEIMIAGSRAFEVSKSPDATGILARLRQFRGRLPAGFKFDRLEANER

Nucleotide

• Length: 231 bp

>AT190261 NZ_CP091210:467050-467280 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG