Detailed information of TA system TA190259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 956161..956743
• Replicon: plasmid pMVF16-1
• Accession: NZ_CP069355
• Organism: Microvirga sp. VF16

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: JO965_RS29915
• Protein ID: WP_203444548.1
• Coordinates: 956161..956511 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: JO965_RS29920
• Protein ID: WP_203444549.1
• Coordinates: 956498..956743 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JO965_RS29895	951771..952898	+	1128	WP_203444545.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JO965_RS29900	952895..953527	+	633	WP_203444546.1	dethiobiotin synthase	-
JO965_RS29905	953536..954807	+	1272	WP_203445551.1	adenosylmethionine--8-amino-7-oxononanoate transaminase	-
JO965_RS29910	954810..955799	+	990	WP_203444547.1	beta-ketoacyl-ACP synthase III	-
JO965_RS29915	956161..956511	-	351	WP_203444548.1	endoribonuclease MazF	Toxin
JO965_RS29920	956498..956743	-	246	WP_203444549.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JO965_RS29925	957305..957574	+	270	WP_203445553.1	hypothetical protein	-
JO965_RS29930	957651..958124	+	474	WP_203445552.1	PIN domain-containing protein	-
JO965_RS29935	958338..959021	+	684	WP_203445554.1	transposase	-
JO965_RS29940	959667..960119	-	453	WP_203444550.1	aspartate 1-decarboxylase	-
JO965_RS29945	960824..961174	+	351	WP_203444551.1	HU family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1403018	1403018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-115)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12733.69 Da
• Isoelectric Point: 8.2646

>T190259 WP_203444548.1 NZ_CP069355:c956511-956161 [Microvirga sp. VF16]
MSSPNPPYCPDEGDLIWINFDPQAGREQAGRRPALVLTPRQYNQSTRLCILCPVTTQVKGYPFEVVVNGAGKTRGAVLAD
HIKSASWTDRAAEFIEMADPAVLADVRAKLKPILKI

Nucleotide

• Length: 351 bp

>T190259 NZ_CP091208:c13563-12700 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGAAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTGTTGTCATTGATAATGATACCTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACACCTTATTCTAATCGTATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGCACAGGACGAACAACAGACGTTCCTATTCAAACAGCCACAATGCTACAAG
CCAAAGGTTATGAAACAAAAACGTACGCCATGGCAGTACCCAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8850.00 Da
• Isoelectric Point: 4.3507

>AT190259 WP_203444549.1 NZ_CP069355:c956743-956498 [Microvirga sp. VF16]
MRVQFAQWGNSLALRIPQAFAREISARPGRQAELTVQGGTLVIEPIDEAPVYTLEELLEGITPENLHGETSMGRAVGNEF
A

Nucleotide

• Length: 246 bp

>AT190259 NZ_CP091208:c13837-13565 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTAAAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA