Detailed information of TA system TA190258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 708487..709049
• Replicon: plasmid pMVF16-1
• Accession: NZ_CP069355
• Organism: Microvirga sp. VF16

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JO965_RS28840
• Protein ID: WP_203444365.1
• Coordinates: 708681..709049 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JO965_RS28835
• Protein ID: WP_203444364.1
• Coordinates: 708487..708684 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JO965_RS28820	704573..705994	+	1422	WP_203444361.1	IS4 family transposase	-
JO965_RS28825	706004..706231	-	228	WP_203444362.1	hypothetical protein	-
JO965_RS28830	706298..707908	-	1611	WP_203444363.1	transposase	-
JO965_RS28835	708487..708684	+	198	WP_203444364.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JO965_RS28840	708681..709049	+	369	WP_203444365.1	type II toxin-antitoxin system VapC family toxin	Toxin
JO965_RS28845	709063..709956	+	894	WP_203444366.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1403018	1403018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-46)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13433.64 Da
• Isoelectric Point: 6.7265

>T190258 WP_203444365.1 NZ_CP069355:708681-709049 [Microvirga sp. VF16]
VIIVDTSVWIDHLKSSDPVLADLLGKGRVLAHSFVTGELALGGLRQRRTVLESLRDLPQATVASDDEVMMLIEREQLYGL
GIGFVDAHLLAAVRLTLGALLWTRDRRLRQAAMRLGLSADFT

Nucleotide

• Length: 369 bp

>T190258 NZ_CP091207:41217-41369 [Enterococcus faecium]
ATTGAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATCTCATCATCATCTTTATAAGGATGGTGTTAGAATCACTGT
TCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGAAAAAAATTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7211.30 Da
• Isoelectric Point: 10.7342

>AT190258 WP_203444364.1 NZ_CP069355:708487-708684 [Microvirga sp. VF16]
VRTTIALDDELVAKAQAFTGLQEKSALVREALKALIERESARRLARLGGTEPDLKPIPRRRTDAQ

Nucleotide

• Length: 198 bp

>AT190258 NZ_CP091207:41403-41834 [Enterococcus faecium]
ATGTTAAGCGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTTTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGAGCTACTTCCAGAACCAACACCAATCAATAAGATCTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCGAATATTTGAAAGAAACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGTTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG