Detailed information of TA system TA190256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 105025..106508
• Replicon: plasmid pMVF16-1
• Accession: NZ_CP069355
• Organism: Microvirga sp. VF16

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: JO965_RS26000
• Protein ID: WP_203445014.1
• Coordinates: 105025..106284 (-)
• Length: 420 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: JO965_RS26005
• Protein ID: WP_203445015.1
• Coordinates: 106281..106508 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JO965_RS25985	102507..102914	-	408	WP_203445013.1	hypothetical protein	-
JO965_RS25990	103053..103382	-	330	WP_203445476.1	helix-turn-helix domain-containing protein	-
JO965_RS25995	103818..104944	-	1127	Protein_109	DUF3800 domain-containing protein	-
JO965_RS26000	105025..106284	-	1260	WP_203445014.1	type II toxin-antitoxin system HipA family toxin	Toxin
JO965_RS26005	106281..106508	-	228	WP_203445015.1	helix-turn-helix domain-containing protein	Antitoxin
JO965_RS26010	106613..107251	+	639	WP_203445016.1	hypothetical protein	-
JO965_RS26015	107461..107718	+	258	Protein_113	transposase	-
JO965_RS26020	107926..108236	-	311	Protein_114	transposase	-
JO965_RS26025	108616..108843	-	228	WP_203445017.1	hypothetical protein	-
JO965_RS26030	108840..109481	-	642	WP_203445018.1	hypothetical protein	-
JO965_RS26035	109478..110413	-	936	WP_203445019.1	pantoate--beta-alanine ligase	-
JO965_RS26040	110423..111304	-	882	WP_203445020.1	3-methyl-2-oxobutanoate hydroxymethyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1403018	1403018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (147-354)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-66)

Sequences

Toxin

Protein

• Length: 420 a.a.
• Molecular weight: 45455.31 Da
• Isoelectric Point: 6.5057

>T190256 WP_203445014.1 NZ_CP069355:c106284-105025 [Microvirga sp. VF16]
MTHLLDVWFGDTKAGILAQDEAGALSYAYDPNYLAGREPRSISFSMPLQEAPYADRVVRPFFSGLLPDEGARQRLAGALG
ISAGNAFGLLEVIGGECAGALSLYRAGEAPPSSDDDGVELLNPARLEEILGKLRERPLLGGEEGVRLSLAGAQDKLAVCV
EGDDIGLAKGGRPTTHILKPFIQALDGTVENELFCLHLAARLKLPVPAVEMRRTGAISFLIIERYDRSPHKGGTITRLHQ
EDFCQALSVPPELKYEEEGGPGTEKALDLIGRATARPAADRLRFLRMLIFHYLVGNADAHGKNYALLYRGKVPDLAPLYD
VVCTAAYPRLAKKLAMAIGGRSVPDTIQLQHWLTLVPETRSAQRLLVKDIAELAGRIRGEADKLLAEFEGAGVTHEILTV
VRRIIEARAAHLLRIATPN

Nucleotide

• Length: 1260 bp

>T190256 NZ_CP091206:c711923-711582 [Enterococcus faecium]
GTGTCAGGAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTTATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8027.26 Da
• Isoelectric Point: 10.4646

>AT190256 WP_203445015.1 NZ_CP069355:c106508-106281 [Microvirga sp. VF16]
MQTVLDSKSFGLIIRQERRSQKLTQEQLAGLTGVGVRFVRELEAGKESCQIGRALQVAAALGLSVSVGSRLESAP

Nucleotide

• Length: 228 bp

>AT190256 NZ_CP091206:c712153-711923 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGACGATGGTACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG