Detailed information of TA system TA190254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1542547..1543111
• Replicon: chromosome
• Accession: NZ_CP069353
• Organism: Saccharopolyspora erythraea strain NRRL 23338

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A4F9B1
• Locus tag: JQX30_RS07110
• Protein ID: WP_009947934.1
• Coordinates: 1542547..1542894 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A4F9B2
• Locus tag: JQX30_RS07115
• Protein ID: WP_009947932.1
• Coordinates: 1542881..1543111 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JQX30_RS07085	1537688..1538260	+	573	WP_009947940.1	siderophore-interacting protein	-
JQX30_RS07090	1538375..1539715	+	1341	WP_021341391.1	lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family protein	-
JQX30_RS07095	1539850..1540908	+	1059	WP_009947937.1	LLM class flavin-dependent oxidoreductase	-
JQX30_RS07100	1541027..1541416	+	390	WP_011873324.1	DUF3817 domain-containing protein	-
JQX30_RS07105	1541413..1542351	+	939	WP_044547166.1	heme o synthase	-
JQX30_RS07110	1542547..1542894	-	348	WP_009947934.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JQX30_RS07115	1542881..1543111	-	231	WP_009947932.1	antitoxin	Antitoxin
JQX30_RS07120	1543232..1543858	-	627	WP_009947931.1	maleylpyruvate isomerase N-terminal domain-containing protein	-
JQX30_RS07125	1544078..1544629	+	552	WP_009947930.1	snapalysin family zinc-dependent metalloprotease	-
JQX30_RS07130	1544831..1546597	+	1767	WP_009947928.1	alpha-L-fucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12642.51 Da
• Isoelectric Point: 11.5554

>T190254 WP_009947934.1 NZ_CP069353:c1542894-1542547 [Saccharopolyspora erythraea]
MRRGDVYWVDFEPIRGSEADKTRPAVVVSNDAANRSAERARRGVITVVPLTSNVNRVLPFQVRLRAADCGLAGDSKAQAE
QVRAVAFHRLGKRIGRVPDTTLAKLDNALRLHLGL

Nucleotide

• Length: 348 bp

>T190254 NZ_CP091202:13466-14329 [Enterococcus hirae]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8319.04 Da
• Isoelectric Point: 3.9693

>AT190254 WP_009947932.1 NZ_CP069353:c1543111-1542881 [Saccharopolyspora erythraea]
MKLSVSLTDEDVTFVDEYAQQVGAPSRSAVIHQAISLLRSAELEDAYASAWAEWESGDDRKLWETTASDGIADASR

Nucleotide

• Length: 231 bp

>AT190254 NZ_CP091202:13192-13464 [Enterococcus hirae]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Structures

Toxin

• AlphaFold DB: A4F9B1

Antitoxin

• AlphaFold DB: A4F9B2